Datasheet

KLRA2 Antibody
CATALOG NUMBER: 5017

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:KLRA2 antibody can be used for detection of KLRA2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1406 - Mouse Spleen Tissue Lysate
IMMUNOGEN:KLRA2 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of mouse KLRA2.

The immunogen is located within the last 50 amino acids of KLRA2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:KLRA2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:KLRA2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KLRA2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:KLRA2 Antibody: Ly49b, Klra30, Ly49-b, Ly49b, Killer cell lectin-like receptor 2, Lymphocyte antigen 49b, Ly-49b
ACCESSION NO.:Q60660
PROTEIN GI NO.:2497636
OFFICIAL SYMBOL:Klra2
GENE ID:16633

Background

BACKGROUND:KLRA2 Antibody: KLRA2 (also known as Ly49B) is a member of the LY49 family of receptors in Natural Killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors), or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylation of several substrates. KLRA2 is thought to be an inhibitory receptor and associates with the protein tyrosine phosphatases SHP1, SHP2, and SHIP in transfected cells. This antibody is predicted to not cross-react with other LY49 receptors.
REFERENCES: 1) Gosselin P, Lusignan Y, Brennan J, et al. The NK2.1 receptor is encoded by Ly-49C and its expression is regulated by MHC class I alleles. Int. Immunol.1997; 533-40.
2) Smith HR, Karlhofer FM, and Yokoyama WM. Ly-49 multigene family expressed by IL-2-activated NK cells. J. Immunol.1994; 153:1068-79.
3) Brennan J, Mager D, Jefferies W, et al. Expression of different members of the Ly-49 gene family defines distinct natural killer cell subsets and cell adhesion properties. J. Exp. Med.1994; 180:2287-95.
4) Saleh A, Davies GE, Pascal V, et al. Identification of probabilistic transcriptional switches in the Ly49 gene cluster: a eukaryotic mechanism for selective gene activation. Immunity2004; 21:55-66.

For Research Use Only