Datasheet

KLRA1 Antibody
CATALOG NUMBER: 4999

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:KLRA1 antibody can be used for detection of KLRA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1406 - Mouse Spleen Tissue Lysate
 2) Cat. No. 17-010 - HEK293 Cell Slide
SPECIFICITY:At least three isoforms of KLRA1 are known to exist; this antibody recognizes only the long isoform.
IMMUNOGEN:KLRA1 antibody was raised against a 13 amino acid synthetic peptide near the center of human KLRA1.

The immunogen is located within amino acids 140 - 190 of KLRA1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:KLRA1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:KLRA1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KLRA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:KLRA1 Antibody: Ly49, KLRA#, KLRA1, LY49L, Ly-49L
ACCESSION NO.:EAW96194
PROTEIN GI NO.:119616600
OFFICIAL SYMBOL:KLRA1P
GENE ID:10748

Background

BACKGROUND:KLRA1 Antibody: KLRA1 (also known as Ly49L) is a member of the LY49 family of receptors in Natural Killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors), or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylation of several substrates. KLRA1 is thought to be an activating receptor, inducing DAP12 phosphorylation in response to antibody-mediated cross-linking of KLRA1 on NK cells.
REFERENCES: 1) Makrigiannis AP, Etzler J, Winkler-Pickett R, et al. Identification of the Ly49L protein: evidence for activating counterparts to inhibitory Ly49 proteins. J. Leuk. Biol.2000; 68:765-71.
2) Smith HR, Karlhofer FM, and Yokoyama WM. Ly-49 multigene family expressed by IL-2-activated NK cells. J. Immunol.1994; 153:1068-79.
3) Brennan J, Mager D, Jefferies W, et al. Expression of different members of the Ly-49 gene family defines distinct natural killer cell subsets and cell adhesion properties. J. Exp. Med.1994; 180:2287-95.

For Research Use Only