Datasheet

KLOTHO Antibody
CATALOG NUMBER: 6107

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:KLOTHO antibody can be used for detection of KLOTHO by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1211 - HepG2 Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 111 kDa

Observed: 106 kDa
SPECIFICITY:Three isoforms of KLOTHO are known to exist.
IMMUNOGEN:KLOTHO antibody was raised against a 16 amino acid synthetic peptide near the center of human KLOTHO.

The immunogen is located within amino acids 410 - 460 of KLOTHO.
HOST SPECIES:Rabbit

Properties

PURIFICATION:KLOTHO Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:KLOTHO Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KLOTHO antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:KLOTHO Antibody: Klotho
ACCESSION NO.:NP_004786
PROTEIN GI NO.:24497614
OFFICIAL SYMBOL:KL
GENE ID:9365

Background

BACKGROUND:KLOTHO Antibody: KLOTHO is the systemic anti-aging hormone within the glycosidase1 superfamily. It encodes a type I membrane protein that is abundant in the kidney and brain. In mice, a deficiency in KLOTHO expression leads to various systemic phenotypes resembling human aging such as arteriosclerosis, osteoporosis, and skin atrophy together with growth retardation, short life-span and infertility. Transgenic mice overexpressing KLOTHO have an extended life span by inhibiting insulin/IGF1 signaling. KLOTHO is involved in the regulation of calcium/phosphorus homeostasis by inhibiting the synthesis of active vitamin D and identified as a potential tumor suppressor.
REFERENCES: 1) Kuro-o M, Matsumura Y, and Aizawa H. Mutation of the mouse klotho gene leads to a syndrome resembling ageing. Nature1997; 390:45-51.
2) Kurosu H, Yamamoto M, Clark JD, et al. Suppression of aging in mice by the hormone Klotho. Science2005; 309:1829-33.
3) Liu H, Fergusson MM, Castilho RM, et al. Augmented Wnt signaling in a mammalian model of accelerated aging. Science 2007; 317:803-6.
4) Wolf I, Levanon-Cohen S, Bose S, et al. Klotho: a tumor suppressor and a modulator of the IGF-1 and FGF pathways in human breast cancer. Oncogene2008; 27:7094-105.

For Research Use Only