KLK4 Antibody Cat. No.: 7445

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: KLK4 antibody was raised against a 15 amino acid peptide near the center of human KLK4.

The immunogen is located within amino acids 80 - 130 of KLK4 .
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: KLK4 antibody can be used for detection of KLK4 by Western blot at 1 - 2 μg/ml.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: KLK4 antibody is human specific. At least three isoforms of KLK4 are known to exist.
POSITIVE CONTROL:1) Cat. No. 1305 - Human Kidney Tissue Lysate
 2) Cat. No. 10-401 - Human Kidney Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 28 kDa

Observed: 31kDa

psi-iconProperties
PURIFICATION:KLK4 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:KLK4 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KLK4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:KLK4
ALTERNATE NAMES:KLK4 Antibody : ARM1, EMSP, PSTS, AI2A1, EMSP1, KLK-L1, PRSS17, kallikrein, Kallikrein-4, Enamel matrix serine proteinase 1
ACCESSION NO.:NP_004908
PROTEIN GI NO.:89142741
GENE ID:9622
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Kallikreins (KLKs) belong to the serine protease family of proteolytic enzymes having diverse physiological functions (1). Kallikrein 4 (KLK4), also known as prostase or EMSP1 (enamel matrix serine protease 1), contains one peptidase S1 domain and the expression in prostate is regulated by hormone (1,2). Many kallikreins are implicated in carcinogenesis and have potential as novel cancer and other disease biomarkers (3). KLK4 may have additional roles such as functioning as one of the two major enamel proteases identified that process enamel matrix proteins. Defects in KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1) (4).
REFERENCES:1) Stephenson SA, Verity K, Ashworth LK, et al. Localization of a new prostate-specific antigen-related serine protease gene, KLK4, is evidence for an expanded human kallikrein gene family cluster on chromosome 19q13.3-13.4. J. Biol. Chem. 1999; 274:23210-4.
2) Myers SA and Clements JA. Kallikrein 4 (KLK4), a new member of the human kallikrein gene family is up-regulated by estrogen and progesterone in the human endometrial cancer cell line, KLE. J. Clin. Endocrinol. Metab. 2001; 86:2323-6.
3) Kontos CK, Chantzis D, Papadopoulos IN, et al. Kallikrein-related peptidase 4 (KLK4) mRNA predicts short-term relapse in colorectal adenocarcinoma patients. Cancer Lett. 2013; 330:106-12
4) Shimizu-Okabe C, Yousef GM, Diamandis EP, et al. Expression of the kallikrein gene family in normal and Alzheimer’s disease. Neuroreport 2001; 12:27447-51.

ANTIBODIES FOR RESEARCH USE ONLY.

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