Datasheet

KLHL1 Antibody
CATALOG NUMBER: 3015

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (100%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:KLHL1 antibody can be used for the detection of KLHL1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 1403 - Mouse Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 82 kDa

Observed: 82 kDa
IMMUNOGEN:KLHL1 antibody was raised against a 14 amino acid synthetic peptide from near the amino-terminus of human KLHL1.

The immunogen is located within the first 50 amino acids of KLHL1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:KLHL1 Antibody is immunoaffinity purified .
PHYSICAL STATE:Liquid
BUFFER:KLHL1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KLHL1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:KLHL1 Antibody: MRP2, KIAA1490, Kelch-like protein 1
ACCESSION NO.:AAF81719
PROTEIN GI NO.:8926179
OFFICIAL SYMBOL:KLHL1
GENE ID:57626

Background

BACKGROUND:KLHL1 Antibody: The mammalian Kelch-like 1 (KLHL1) was initially discovered as a homolog to the Drosophila Kelch gene that is highly expressed in several brain tissues. The predicted protein domain structure of KLHL1 is characteristic of a number of proteins that bind actin, form dimers, and often act as actin-organizing proteins. Based on the presence of anti-sense RNA that spans the transcription and translation start sites as well as the first splice site of KLHL1 in brain tissue of individuals suffering from the neurodegenerative disorder spinocerebellar ataxia type 8 (SCA8), it has been suggested that KLHL1 is involved this disease and that regulation of KLHL1 protein may be affected by antisense RNA expression.
REFERENCES: 1) Nemes JP, Benzo KA, and Koob MD. The SCA8 transcript is an antisense RNA to a brain-specific transcript encoding a novel actin-binding protein (KLHL1). Human Mol. Gen. 2000; 9:1543-51.
2) Adams J, Kelso R, and Cooley L. The kelch repeat superfamily of proteins: propellers of cell function. Trends Cell Biol. 2000; 10:17-24.
3) Robinson DN and Cooley L. Drosophila kelch is an oligomeric ring canal actin organizer. J. Cell Biol. 1997; 138:799-810.

For Research Use Only