Datasheet

KLF4 Antibody
CATALOG NUMBER: 5603

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Pig: (80%), Bovine: (74%), Chicken: (75%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:KLF4 antibody can be used for detection of KLF4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1304 - Human Liver Tissue Lysate
IMMUNOGEN:KLF4 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human KLF4.

The immunogen is located within the last 50 amino acids of KLF4.
HOST SPECIES:Rabbit

Properties

PURIFICATION:KLF4 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:KLF4 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KLF4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:KLF4 Antibody: EZF, GKLF, EZF, Krueppel-like factor 4, Epithelial zinc finger protein EZF
ACCESSION NO.:AAH30811
PROTEIN GI NO.:21410813
OFFICIAL SYMBOL:KLF4
GENE ID:9314

Background

BACKGROUND:KLF4 Antibody: KLF4 is a transcription factor that functions as both a transcriptional activator and repressor to regulate proliferation and differentiation of multiple cell types. The role of KLF4 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing KLF4 and the transcription factors POU5F1, Sox2, and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing KLF4, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. KLF4 interacts directly with POU5F1 and Sox2 in iPS and ES cells and activates the target gene NANOG.
REFERENCES: 1) Evans PM, Zhang W, Chen X, et al. Kruppel-like factor 4 is acetylated by p300 and regulates gene transcription via modulation of histone acetylation. J. Bio. Chem.2007; 282:33994-4002.
2) Carpenter MK, Rosler E, and Rao MS. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells2003; 5:79-88.
3) Takahashi K and Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell2006; 1263:663-76.
4) Okita K, Nakagawa M, Hyenjong H, et al. Generation of mouse induced pluripotent stem cells without viral vectors. Science2008; 322:949-53.

For Research Use Only