Datasheet

KLF17 Antibody
CATALOG NUMBER: 8041

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Pig: (81%), Chicken: (79%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:KLF17 antibody can be used for detection of KLF17 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1464 - Rat Liver Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 28, 39, 43 kDa

Observed: 38 kDa
SPECIFICITY:KLF17 antibody is human, mouse and rat reactive. At least two isoforms of KLF17 are known to exist; this antibody will only detect the large isoform. KLF17 antibody is predicted to not cross-react with other KLF proteins.
IMMUNOGEN:KLF17 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human KLF17.

The immunogen is located within the last 50 amino acids of KLF17.
HOST SPECIES:Rabbit

Properties

PURIFICATION:KLF17 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:KLF17 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KLF17 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Kruppel-like factor 17, zinc finger protein 393, ZNF393, Zfp393
ACCESSION NO.:NP_775755
PROTEIN GI NO.:104294874
OFFICIAL SYMBOL:KLF17
GENE ID:128209

Background

BACKGROUND:KLF17, also known as ZNF393, is a member of the Sp/KLF family of transcription factors (1,2). KLF17 binds to the promoter and negatively regulates the transcription of Id-1, a key metastasis regulator in breast cancer (3). Suppression of KLF17 promotes breast cancer cell invasion and epithelial-mesenchymal transition (3). Down-regulated KLF17 expression is also associated tumor invasion and poor prognosis in hepatocellular carcinoma, suggesting that the level of KLF17 expression can serve as a prognostic indicator (4).
REFERENCES: 1) Yan W, Burns KH, Ma L, et al. Identification of Zfp393, a germ cell-specific gene encoding a novel zinc finger protein. Mech. Dev. 2002; 118:233-9.
2) van Vliet J, Crofts LA, Quinlan KG, et al. Human KLF17 is a new member of the Sp/KLF family of transcription factors. Genomics 2006; 87:474-82.
3) Gumireddy K, Li A, Gimotty PA, et al. KLF17 is a negative regulator of epithelial-mesenchymal transition and metastasis in breast cancer. Nat. Cell Biol. 2009; 11:1297-304.
4) Liu FY, Deng YL, Li Y, et al. Down-regulated KLF17 expression is associated with tumor invasion and poor prognosis in hepatocellular carcinoma. Med. Oncol. 2013; 30:425.

For Research Use Only