KIRREL3 Antibody Cat. No.: 7973

Availability: In stock

$0.00

 Price Options

$0.00
psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: KIRREL3 antibody was raised against a 19 amino acid peptide near the amino terminus of human KIRREL3.

The immunogen is located within amino acids 20 - 70 of KIRREL3.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: KIRREL3 antibody can be used for detection of KIRREL3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
SPECIFICITY: KIRREL3 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. KIRREL3 antibody is predicted to not cross-react with other members of the KIRREL protein family.
POSITIVE CONTROL:1) Cat. No. 1405 - Mouse Kidney Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 64, 84 kDa

Observed: 60 kDa

psi-iconProperties
PURIFICATION:KIRREL3 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:KIRREL3 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KIRREL3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:KIRREL3
ALTERNATE NAMES:Kin of irregular chiasm-like protein 3, Kin of IRRE-like protein 3, MRD4, KIRRE, NEPH2, PRO4502, Nephrin-like protein 2
ACCESSION NO.:NP_115920
PROTEIN GI NO.:26006461
GENE ID:84623
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:KIRREL3, also known as nephrin-like protein 2, is a type I transmembrane protein belonging to a family of three podocin interacting proteins and the immunoglobulin superfamily (1). KIRREL3 is involved in the regulation of both glomerular and neural development (2), and more specifically, the nucleogenesis of the pontine nuclei in the developing hindbrain (3). KIRREL3 has also been shown to interact with the synaptic scaffold protein calmodulin-associated serine/threonine kinase (CASK) in neuronal cells (4).
REFERENCES:1) Sellin L, Huber TB, Gerke P, et al. NEPH1 defines a novel family of podocin interacting proteins. FASEB J. 2003; 17:115-7.
2) Neumann-Haefelin E, Kramer-Zucker A, Slanchev K, et al. A model organism approach: defining the role of Neph proteins as regulators of neuron and kidney morphogenesis. Hum. Mol. Genet. 2010; 19:2347-59.
3) Nishida K, Nakayama K, Yoshimura S, et al. Role of Neph2 in pontine nuclei formation in the developing hindbrain. Mol. Cell Neurosci. 2011; 46:662-70.
4) Mizuhara E, Minaki Y, Nakatani T, et al. Purkinje cells originate from cerebellar ventricular zone progenitors positive for Neph3 and E-cadherin. Dev. Biol. 2010; 338:202-14.

ANTIBODIES FOR RESEARCH USE ONLY.

For additional information, visit ProSci's Terms & Conditions Page.