KIRREL2 Antibody Cat. No.: 7971

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: KIRREL2 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human KIRREL2.

The immunogen is located within amino acids 550 - 600 of KIRREL2.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: KIRREL2 antibody can be used for detection of KIRREL2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: KIRREL2 antibody is human, mouse and rat reactive. At least five isoforms are are known to exist. This antibody is predicted to not cross-react with other members of the KIRREL protein family.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
 2) Cat. No. 11-101 - Human Pancreas Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 64, 70, 78 kDa

Observed: 70 kDa

psi-iconProperties
PURIFICATION:KIRREL2 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:KIRREL2 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KIRREL2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:KIRREL2
ALTERNATE NAMES:Kin of irregular chiasm-like protein 2, Kin of IRRE-like protein 2, NEPH3, Nephrin-like protein 3, NLG1, FILTRIN
ACCESSION NO.:NP_954649
PROTEIN GI NO.:145275174
GENE ID:84063
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:KIRREL2, also known as nephrin-like 3, is a type I transmembrane protein belonging to the immunoglobulin superfamily of cell adhesion molecules (1). KIRREL2 consists of five extracellular Ig-like repeats, a transmembrane domain, several glycosylation sites and a cytoplasmic domain that has a stretch of nine conserved residues (1,2). KIRREL2 has been found to be expressed in beta cells of pancreatic islets and is involved in the regulation of both glomerular and neural development (3,4). Downregulation of KIRREL2 is implicated in several proteinuric diseases (4,5).
REFERENCES:1) Sun C, Kilburn D, Lukashin A, et al. KIRREL2, a novel immunoglobulin superfamily gene expressed primarily in beta cells of the pancreatic islets. Genomics 2003; 82:130-42.
2) Heikkila E, Ristola M, Havana M, et al. Trans-interaction of nephrin and Neph1/Neph3 induces cell adhesion that associates with decreased tyrosine phosphorylation of nephrin. Biochem. J. 2011; 435:619-28.
3) Wang H, Lehtonen S, Chen YC, et al. Neph3 associates with regulation of glomerular and neural development in zebrafish. Differentiation 2012; 83:38-46.
4) Mizuhara E, Minaki Y, Nakatani T, et al. Purkinje cells originate from cerebellar ventricular zone progenitors positive for Neph3 and E-cadherin. Dev. Biol. 2010; 338:202-14.

ANTIBODIES FOR RESEARCH USE ONLY.

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