Datasheet

KINDLIN3 Antibody
CATALOG NUMBER: 4797

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:KINDLIN3 antibody can be used for detection of KINDLIN3 by Western blot at 1 and 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1466 - Rat Spleen Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 73 kDa

Observed: 73 kDa
IMMUNOGEN:KINDLIN3 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of the human KINDLIN3.

The immunogen is located within the last 50 amino acids of KINDLIN3.
HOST SPECIES:Rabbit

Properties

PURIFICATION:KINDLIN3 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:KINDLIN3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KINDLIN3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:KINDLIN3 Antibody: URP2, KIND3, MIG-2, MIG2B, URP2SF, UNC112C, URP2, Fermitin family homolog 3, Kindlin-3
ACCESSION NO.:NP_848537
PROTEIN GI NO.:41281905
OFFICIAL SYMBOL:FERMT3
GENE ID:83706

Background

BACKGROUND:KINDLIN3 Antibody: The three KINDLINs are a novel family of focal adhesion proteins, localizing to integrin adhesion sites. The KINDLIN proteins are composed of a centrally located FERM domain interrupted by a pleckstrin homology (PH) domain. KINDLIN1 and KINDLIN2 have been shown to play an essential role in integrin-mediated adhesion and spreading. In contrast to the widely expressed KINDLIN1 and KINDLIN2, KINDLIN3 is restricted to hematopoietic cells and is particularly abundant in megakaryocytes and platelets. Several reports describe a transcriptional misregulation of KINDLINs in various types of cancer. A recent study demonstrates that KINDLIN3 is essential for platelet integrin activation and subsequent integrin outside-in signaling, suggesting it may serve as a potential target for the design of therapeutics aimed at specifically disrupting integrin activation in platelets and leukocytes.
REFERENCES: 1) Ussar S, Wang HV, Linder S, et al. The Kindlins: subcellular localization and expression during murine development. Exp. Cell Res.2006; 312:3142-51.
2) Weinstein EJ, Bourner M, Head R, et al. URP1: a member of a novel family of PH and FERM domain-containing membrane-associated proteins is significantly over-expressed in lung and colon carcinomas. Biochim. Biophys. Acta2003; 1637:207-16.
3) Boyd RS, Adam PJ, Patel S, et al. Proteomic analysis of the cell-surface membrane in chronic lymphocytic leukemia: identification of two novel proteins, BCNP1 and MIG2B. Leukemia2003; 17:1605-12.
4) Mory A, Feigelson SW, Yarali N, et al. Kindlin-3: a new gene involved in the pathogenesis of LAD-III. Blood2008; 112:2591.

For Research Use Only