KHDC1 Antibody Cat. No.: 7423

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: KHDC1 antibody was raised against a 15 amino acid peptide near the amino terminus of human KHDC1.

The immunogen is located within amino acids 30 - 80 of KHDC1.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: KHDC1 antibody can be used for detection of KHDC1 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in rat samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
SPECIFICITY: KHDC1 antibody is human, mouse and rat reactive. At least three isoforms of KHDC1 are known to exist; this antibody will only detect the longest isoform.
POSITIVE CONTROL:1) Cat. No. 1464 - Rat Liver Tissue Lysate
 2) Cat. No. 10-201 - Human Liver Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 18 kDa

Observed: 26 kDa

psi-iconProperties
PURIFICATION:KHDC1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:KHDC1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KHDC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:KHDC1
ALTERNATE NAMES:KHDC1 Antibody: NDG1, C6orf148, bA257K9.4, KH homology domain-containing protein 1
ACCESSION NO.:NP_085045
PROTEIN GI NO.:117956389
GENE ID:80759
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:KHDC1 Antibody: KHDC1 family members are K-homology (KH) domain-containing RNA binding proteins that are involved in various aspects of RNA metabolism, ranging from transcription to RNA splicing, transportation, translation, and stability (1). KHDC1 is highly expressed in oocytes and interacts with cytoplasmic polyadenylation element-binding protein 1 (CPEB1), a key translational regulator controlling the polyA length of mRNAs (2,3). KHDC1 functions as a global translational repressor and induces apoptosis through an ER-dependent signaling pathway and that the C-terminal putative trans-membrane motif (TMM) is critical for its activities (3,4).
REFERENCES:1) Valverde R, Edwards L, and Regan L. Structure and function of KH domains. FEBS J. 2008; 275:2712–26.
2) Pierre A, Gautier M, Callebaut I, et al. Atypical structure and phylogenomic evolution of the new eutherian oocyte- and embryo-expressed KHDC1/DPPA5/ECAT1/OOEP gene family. Genomics 2007; 90:583-94.
4) Cai C, Liu J, Wang C, et al. KHDC1A, a novel translational repressor, induces endoplasmic reticulum-dependent apoptosis. DNA Cell Biol. 2012; 31:1447-57.

ANTIBODIES FOR RESEARCH USE ONLY.

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