Datasheet

KHDC1 Antibody
CATALOG NUMBER: 7423

Availability: In stock

$0.00
Product Price Options
$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:KHDC1 antibody can be used for detection of KHDC1 by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1464 - Rat Liver Tissue Lysate
 2) Cat. No. 10-201 - Human Liver Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 18 kDa

Observed: 26 kDa
SPECIFICITY:KHDC1 antibody is human, mouse and rat reactive. At least three isoforms of KHDC1 are known to exist; this antibody will only detect the longest isoform.
IMMUNOGEN:KHDC1 antibody was raised against a 15 amino acid peptide near the amino terminus of human KHDC1.

The immunogen is located within amino acids 30 - 80 of KHDC1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:KHDC1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:KHDC1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KHDC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:KHDC1 Antibody: NDG1, C6orf148, bA257K9.4, KH homology domain-containing protein 1
ACCESSION NO.:NP_085045
PROTEIN GI NO.:117956389
OFFICIAL SYMBOL:KHDC1
GENE ID:80759

Background

BACKGROUND:KHDC1 Antibody: KHDC1 family members are K-homology (KH) domain-containing RNA binding proteins that are involved in various aspects of RNA metabolism, ranging from transcription to RNA splicing, transportation, translation, and stability (1). KHDC1 is highly expressed in oocytes and interacts with cytoplasmic polyadenylation element-binding protein 1 (CPEB1), a key translational regulator controlling the polyA length of mRNAs (2,3). KHDC1 functions as a global translational repressor and induces apoptosis through an ER-dependent signaling pathway and that the C-terminal putative trans-membrane motif (TMM) is critical for its activities (3,4).
REFERENCES: 1) Valverde R, Edwards L, and Regan L. Structure and function of KH domains. FEBS J. 2008; 275:2712–26.
2) Pierre A, Gautier M, Callebaut I, et al. Atypical structure and phylogenomic evolution of the new eutherian oocyte- and embryo-expressed KHDC1/DPPA5/ECAT1/OOEP gene family. Genomics 2007; 90:583-94.
4) Cai C, Liu J, Wang C, et al. KHDC1A, a novel translational repressor, induces endoplasmic reticulum-dependent apoptosis. DNA Cell Biol. 2012; 31:1447-57.

For Research Use Only