KEAP1 Antibody Cat. No.: 7045

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Pig: (100%)
IMMUNOGEN: Anti-KEAP1 antibody (7045) was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human KEAP1.

The immunogen is located within the last 50 amino acids of KEAP1.
TESTED APPLICATIONS: ELISA, IF, WB
APPLICATIONS: WB: 1-2 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: At least two isoforms of KEAP1 are known to exist.
POSITIVE CONTROL:1) Cat. No. 1302 - Human Lung Tissue Lysate
 2) Cat. No. 10-101 - Human Lung Tissue Slide
 3) Cat. No. 1224 - Daudi Cell Lysate
 4) Cat. No. 1204– K562 Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 70kD

Observed: 65-70kD
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar KEAP1 expression profile in human, mouse (3T3/NIH) and rat (YB2/0) cell lines detected by two independent anti-KEAP1 antibodies that recognize different epitopes, 7045 against C-terminus domain and 22-002 against recombinant fusion protein of human KEAP1.  KEAP1 proteins are detected in all the tested cell lines at different expression levels by the two independent antibodies.  

KO Validation (Figure 3): Anti-KEAP1 antibodies (7045) specificity was further verified by KEAP1 specific knockout. KEAP1 signal was not detected in KEAP1 knockout HeLa cells as compared to that in control wild type cells.

ISOFORMS: Human KEAP1 has 1 isoform, (624aa, 69.7kD). Mouse KEAP1 has 1 isoform (624aa, 69.6kD). Rat KEAP1 has only one isoform (624aa, 69.4 kD). This antibody detects KEAP1 of human, mouse and rat.

psi-iconProperties
PURIFICATION:KEAP1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:KEAP1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KEAP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:KEAP1
ALTERNATE NAMES:KEAP1 Antibody: INrf2, KLHL19, INRF2, KIAA0132, Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, INrf2
ACCESSION NO.:NP_987096
PROTEIN GI NO.:45269145
GENE ID:9817
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:KEAP1 Antibody: KEAP1 (kelch-like ECH-associated protein 1) is a stress sensing adaptor for the Cullin3 (Cul3)-dependent E3 ubiquitin ligase complex that negatively regulates NRF2 (NF-E2-related factor 2) and plays a role in the oxidative stress response. It targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome. KEAP1 contains an amino terminal BTB/POZ domain and a carboxyl terminal KELCH domain which are required for interaction with NRF2, and in binding Cul3-E3 ubiquitin ligase. Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD). KEAP1 also targets the down regulation of NF-κB activity by targeting IKKβ degradation. Mutation of the KEAP1 gene is found in lung cancer.
REFERENCES:1) Zhang et al. Mol. Cell. Biol. 2004;24:10941-53.
2) Kobayashi et al. Mol. Cell. Biol. 2004; 24:7130-9.
3) Jiang et al. Int. J. Mol. Med. 2012; 29:946-56.
4) Devling et al. Proc. Natl. Acad. Sci. USA 2005; 102: 7280-5A.

ANTIBODIES FOR RESEARCH USE ONLY.

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