| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Pig: (100%) |
| IMMUNOGEN: | Anti-KEAP1 antibody (7045) was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human KEAP1. The immunogen is located within the last 50 amino acids of KEAP1. |
| TESTED APPLICATIONS: | ELISA, IF, WB |
| APPLICATIONS: | KEAP1 antibody can be used for detection of KEAP1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
| SPECIFICITY: | At least two isoforms of KEAP1 are known to exist. |
| POSITIVE CONTROL: | 1) Cat. No. 1302 - Human Lung Tissue Lysate |
| 2) Cat. No. 10-101 - Human Lung Tissue Slide | |
| 3) Cat. No. 1224 - Daudi Cell Lysate | |
| 4) Cat. No. 1204– K562 Cell Lysate | |
| PREDICTED MOLECULAR WEIGHT: | 69 kDa |
Specifications| VALIDATION: | Independent Antibody Validation in Cell lines (Figure 2) shows similar KEAP1 expression profile in human, mouse (3T3/NIH) and rat (YB2/0) cell lines detected by two independent anti-KEAP1 antibodies that recognize different epitopes, 7045 against C-terminus domain and 22-002 against recombinant fusion protein of human KEAP1. KEAP1 proteins are detected in all the tested cell lines at different expression levels by the two independent antibodies. |
| ISOFORMS: | Human KEAP1 has 1 isoform, (624aa, 69.7kD). Mouse KEAP1 has 1 isoform (624aa, 69.6kD). Rat KEAP1 has only one isoform (624aa, 69.4 kD). This antibody detects KEAP1 of human, mouse and rat. |
| PURIFICATION: | KEAP1 Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | KEAP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | KEAP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | KEAP1 |
| ALTERNATE NAMES: | KEAP1 Antibody: INrf2, KLHL19, INRF2, KIAA0132, Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, INrf2 |
| ACCESSION NO.: | NP_987096 |
| PROTEIN GI NO.: | 45269145 |
| GENE ID: | 9817 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | KEAP1 Antibody: KEAP1 (kelch-like ECH-associated protein 1) is a stress sensing adaptor for the Cullin3 (Cul3)-dependent E3 ubiquitin ligase complex that negatively regulates NRF2 (NF-E2-related factor 2) and plays a role in the oxidative stress response. It targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome. KEAP1 contains an amino terminal BTB/POZ domain and a carboxyl terminal KELCH domain which are required for interaction with NRF2, and in binding Cul3-E3 ubiquitin ligase. Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD). KEAP1 also targets the down regulation of NF-κB activity by targeting IKKβ degradation. Mutation of the KEAP1 gene is found in lung cancer. |
| REFERENCES: | 1) Zhang DD, Lo SC, Cross JV, et al. Keap1 is a redox-regulated substrate adaptor protein for a Cul3-dependent ubiquitin ligase complex. Mol. Cell. Biol. 2004;24:10941-53. | 2) Kobayashi A, Kang MI, Okawa H, et al. Oxidative stress sensor Keap1 functions as an adaptor for Cul3-based E3 ligase to regulate proteasomal degradation of Nrf2. Mol. Cell. Biol. 2004; 24:7130-9. | 3) Jiang J, Mo ZC, Yin K, et al. Epigallocatechin-3-gallate prevents TNF-α-induced NF-kappaB activation thereby upregulating ABCA1 via the Nrf2/Keap1 pathway in macrophage foam cells. Int. J. Mol. Med. 2012; 29:946-56. | 4) Devling TW, Lindsay CD, McLellan LI, et al. Utility of siRNA against Keap1 as a strategy to stimulate a cancer chemopreventive phenotype. Proc. Natl. Acad. Sci. USA 2005; 102: 7280-5A. |
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