KEAP1 Antibody Cat. No.: 7045

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Pig: (100%)
IMMUNOGEN: Anti-KEAP1 antibody (7045) was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human KEAP1.

The immunogen is located within the last 50 amino acids of KEAP1.
TESTED APPLICATIONS: ELISA, IF, WB
APPLICATIONS: KEAP1 antibody can be used for detection of KEAP1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: At least two isoforms of KEAP1 are known to exist.
POSITIVE CONTROL:1) Cat. No. 1302 - Human Lung Tissue Lysate
 2) Cat. No. 10-101 - Human Lung Tissue Slide
 3) Cat. No. 1224 - Daudi Cell Lysate
 4) Cat. No. 1204– K562 Cell Lysate
PREDICTED MOLECULAR WEIGHT: 69 kDa
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar KEAP1 expression profile in human, mouse (3T3/NIH) and rat (YB2/0) cell lines detected by two independent anti-KEAP1 antibodies that recognize different epitopes, 7045 against C-terminus domain and 22-002 against recombinant fusion protein of human KEAP1.  KEAP1 proteins are detected in all the tested cell lines at different expression levels by the two independent antibodies.  

ISOFORMS: Human KEAP1 has 1 isoform, (624aa, 69.7kD). Mouse KEAP1 has 1 isoform (624aa, 69.6kD). Rat KEAP1 has only one isoform (624aa, 69.4 kD). This antibody detects KEAP1 of human, mouse and rat.

psi-iconProperties
PURIFICATION:KEAP1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:KEAP1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KEAP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:KEAP1
ALTERNATE NAMES:KEAP1 Antibody: INrf2, KLHL19, INRF2, KIAA0132, Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, INrf2
ACCESSION NO.:NP_987096
PROTEIN GI NO.:45269145
GENE ID:9817
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:KEAP1 Antibody: KEAP1 (kelch-like ECH-associated protein 1) is a stress sensing adaptor for the Cullin3 (Cul3)-dependent E3 ubiquitin ligase complex that negatively regulates NRF2 (NF-E2-related factor 2) and plays a role in the oxidative stress response. It targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome. KEAP1 contains an amino terminal BTB/POZ domain and a carboxyl terminal KELCH domain which are required for interaction with NRF2, and in binding Cul3-E3 ubiquitin ligase. Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD). KEAP1 also targets the down regulation of NF-κB activity by targeting IKKβ degradation. Mutation of the KEAP1 gene is found in lung cancer.
REFERENCES:1) Zhang DD, Lo SC, Cross JV, et al. Keap1 is a redox-regulated substrate adaptor protein for a Cul3-dependent ubiquitin ligase complex. Mol. Cell. Biol. 2004;24:10941-53.
2) Kobayashi A, Kang MI, Okawa H, et al. Oxidative stress sensor Keap1 functions as an adaptor for Cul3-based E3 ligase to regulate proteasomal degradation of Nrf2. Mol. Cell. Biol. 2004; 24:7130-9.
3) Jiang J, Mo ZC, Yin K, et al. Epigallocatechin-3-gallate prevents TNF-α-induced NF-kappaB activation thereby upregulating ABCA1 via the Nrf2/Keap1 pathway in macrophage foam cells. Int. J. Mol. Med. 2012; 29:946-56.
4) Devling TW, Lindsay CD, McLellan LI, et al. Utility of siRNA against Keap1 as a strategy to stimulate a cancer chemopreventive phenotype. Proc. Natl. Acad. Sci. USA 2005; 102: 7280-5A.

ANTIBODIES FOR RESEARCH USE ONLY.

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