KDM1B Antibody Cat. No.: 7535

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
IMMUNOGEN: KDM1B antibody was raised against a 17 amino acid peptide near the amino terminus of human KDM1B.

The immunogen is located within amino acids 80 - 130 of KDM1B.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: KDM1B antibody can be used for detection of KDM1B by Western blot at 0.5 μg/ml.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
SPECIFICITY: KDM1B antibody is human, mouse and rat reactive. At least two isoforms of KDM1B are known to exist; this KDM1B antibody will detect both isoforms.
POSITIVE CONTROL:1) Cat. No. 1204 - K562 Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 90 kDa

Observed: 90 kDa

psi-iconProperties
PURIFICATION:KDM1B antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:KDM1B antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KDM1B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:KDM1B
ALTERNATE NAMES:KDM1B Antibody: AOF1, LSD2, C6orf193, bA204B7.3, dJ298J15.2, AOF1, Lysine-specific histone demethylase 1B, Flavin-containing amine oxidase domain-containing protein 1
ACCESSION NO.:NP_694587
PROTEIN GI NO.:116256451
GENE ID:221656
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Flavin-dependent histone demethylases regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (reviewed in 1). KDM1B is a recently identified histone H3K4 demethylase that is required to establish maternal genomic imprints; targeted disruption of the KDM1B gene in mice led to lethality in their embryos, suggesting demethylation of H3K4 is critical for establishing the DNA methylation imprints (2). KDM1B has also been shown to play a role in the transcription elongation in several genes (3).
REFERENCES:1) Culhane JC and Cole PA. LSD1 and the chemistry of histone demethylation. Curr. Opin. Chem. Biol. 2007; 11:561-8.
2) Ciccone DN, Su H, Hevi S, et al. KDM1B is a histone H3K4 demethylase required to establish maternal genomic imprints. Nature 2009; 461:415-8.
3) Fang R, Barbera A, Xu Y, et al. Human LSD2/KDM1b/AOF1 regulates genes transcription by modulating intragenic H3K4me2 methylation. Mol. Cell 2010; 39:222-233.

ANTIBODIES FOR RESEARCH USE ONLY.

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