KCNK12 Antibody Cat. No.: 6425

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: KCNK12 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human KCNK12.

The immunogen is located within amino acids 320 - 370 of KCNK12.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: KCNK12 antibody can be used for detection of KCNK12 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
SPECIFICITY: KCNK12 antibody is predicted to not cross-react with other KCNK protein family members.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate

psi-iconProperties
PURIFICATION:KCNK12 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:KCNK12 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KCNK12 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:KCNK12
ALTERNATE NAMES:KCNK12 Antibody: THIK2, THIK-2, K2p12.1, Potassium channel subfamily K member 12, Tandem pore domain halothane-inhibited potassium channel 2
ACCESSION NO.:NP_071338
PROTEIN GI NO.:11545761
GENE ID:56660
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:KCNK12 Antibody: KCNK13 and KCNK12 (also known as THIK1 and 2) are the first two members of a novel two pore-forming P domains K+ channels subfamily. The pore loop domain, a highly conserved region common to all potassium channels, is involved in determining potassium ion selectivity. Members of this family are all characterized by four transmembrane domains and may function to help influence the resting membrane potential of cells. KCNK12 is expressed mainly in the brain and lung, but also observed in the kidneys, heart and skeletal muscle. KCNK12 is closely related to KCNK13 (58% identity at the amino acid level), but could not yet been functionally expressed in vitro and may require other proteins to become active.
REFERENCES:1) Rajan S, Wischmeyer E, Karschin C, et al. THIK-1 and THIK-2, a novel subfamily of tandem pore domain K+ channels. J. Biol. Chem. 2001; 276:7302-11
2) Jezzini SH and Moroz LL. Identification and distribution of a twopore domain potassium channel in the CNS of Aplysia californica. Brain Res. Mol. Brain Res. 2004; 127:27-38
3) Theilig F, Goranova I, Hirsch JR, et al. Cellular localization of THIK-1 (K(2P)13.1) and THIK-2 (K(2P)12.1) K channels in the mammalian kidney. Cell Physiol. Biochem. 2008; 21:63-74.
4) Girard C, Duprat F, Terrenoire C, et al. Genomic and functional characteristics of novel human pancreatic 2P domain K+ channels. Biochem. Biophys. Res. Commun. 2001; 282:249-56.

ANTIBODIES FOR RESEARCH USE ONLY.

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