KCNK1 Antibody Cat. No.: 8017

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rabbit: (100%), Bovine: (100%), Guinea pig: (100%)
IMMUNOGEN: KCNK1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human KCNK1.

The immunogen is located within amino acids 220 - 270 of KCNK1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: KCNK1 antibody can be used for detection of KCNK1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
SPECIFICITY: KCNK1 antibody is human, mouse and rat reactive. KCNK1 antibody is predicted to not cross-react with other KCNK proteins.
POSITIVE CONTROL:1) Cat. No. 1212 - 3T3 Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 37 kDa

Observed: 35 kDa

psi-iconProperties
PURIFICATION:KCNK1 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:KCNK1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KCNK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:KCNK1
ALTERNATE NAMES:Potassium channel subfamily K member 1, DPK, HOHO, K2P1, K2p1.1, KCNO1, TWIK-1, TWIK1
ACCESSION NO.:NP_002236
PROTEIN GI NO.:4504847
GENE ID:3775
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The potassium channel, subfamily K, member 1 (KCNK1), is member of the superfamily of potassium channel proteins containing two pore-forming P domains (1,2). KCNK1 is a ubiquitous human weakly inward rectifying K+ channel that is widely expressed in human tissues, and particularly abundant in brain and heart (1). Both KCNK1 and the related protein KCNK2 contribute significantly to the passive conductance of hippocampal astrocytes (3).
REFERENCES:1) Lesage F, Guillemare E, Fink M, et al. TWIK-1, a ubiquitous human weakly inward rectifying K+ channel with a novel structure. EMBO J. 1996; 15:1004-11.
2) Goldstein SA, Bockenhauer D, O’Kelly I, et al. Potassium leak channels and the KCNK family of two-P domain subunits. Nat. Rev. Neurosci. 2001; 2:175-84.
3) Zhou M, Xu G, Xie M, et al. TWIK-1 and TREK-2 are potassium channels contributing significantly to astrocyte passive conductance in rat hippocampal slides. J. Neurosci. 2009; 29:8551-64.

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