Datasheet

KappaB ras2 Antibody
CATALOG NUMBER: 2495

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Chicken: (93%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:KappaB ras2 antibody can be used for detection of KappaB ras1 by Western blot at 1 μg/mL.

Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1283 - RAW 264.7 Cell Lysate
IMMUNOGEN:KappaB ras2 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human KappaB ras2.

The immunogen is located within the last 50 amino acids of KappaB ras2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:KappaB ras2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:KappaB ras2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:KappaB ras2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:KappaB ras2 Antibody: KBRAS2, kappaB-Ras2, KBRAS2, NF-kappa-B inhibitor-interacting Ras-like protein 2, I-kappa-B-interacting Ras-like protein 2, Kappa B-Ras protein 2
ACCESSION NO.:NP_060065
PROTEIN GI NO.:19072794
OFFICIAL SYMBOL:NKIRAS2
GENE ID:28511

Background

BACKGROUND:KappaB ras2 Antibody: KappaB ras-1 (κB-ras-1) and kappaB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with IκB (IκB), an inhibitor of the transcription factor NF-κB. IκB exists in two homologous forms, IκB-alpha and IκB-beta, although IκB-beta contains a unique 47-amino acid region within its ankyrin domain. While inactive IκB-alpha-NF-κB complexes can shuttle in and out of the nucleus, IκB-beta-NF-κB complexes are retained exclusively in the cytoplasm. It is suggested that kappaB-ras proteins preferentially bind to the IκB-beta form through this unique insert within the ankyrin region, thus modulating the cellular location of IκB-beta and regulating the rate of degradation of IκB-beta. This antibody is specific for kappaB-ras2 and has no cross-reactivity to kappaB-ras1.
REFERENCES: 1) Fenwick C, Na SY, Voll RE, et al. A subclass of Ras proteins that regulate the degradation of IkappaB. Science 2000; 287:869-73.
2) Chen Y, Wu J and Ghosh G. KappaB-Ras binds to the unique insert within the ankyrin repeat domain if IkappaBbeta and regulates cytoplasmic retention of IkappaBbeta x NF-kappaB complexes. J. Biol. Chem.2003; 278:23101-6.

For Research Use Only