| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Chicken: (94%) |
| IMMUNOGEN: | KappaB ras antibody was raised against a 18 amino acid synthetic peptide from near the center of human KappaB ras 1. The immunogen is located within amino acids 80 - 130 of KappaB ras. |
| TESTED APPLICATIONS: | ELISA, IHC-P, WB |
| APPLICATIONS: | KappaB ras antibody can be used for detection of KappaB ras by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested. |
| POSITIVE CONTROL: | 1) Cat. No. 1283 - RAW 264.7 Cell Lysate |
| 2) Cat. No. 1369 - Human Lymph node Tissue Lysate | |
| 3) Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide |
Specifications| PURIFICATION: | KappaB ras Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | KappaB ras Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | batch dependent |
| STORAGE CONDITIONS: | KappaB ras antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | NKIRAS1 |
| ALTERNATE NAMES: | KappaB ras Antibody: KBRAS1, kappaB-Ras1, KBRAS1, NF-kappa-B inhibitor-interacting Ras-like protein 1, I-kappa-B-interacting Ras-like protein 1, Kappa B-Ras protein 1 |
| ACCESSION NO.: | AAF34998 |
| PROTEIN GI NO.: | 7008402 |
| GENE ID: | 28512 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | KappaB ras Antibody: KappaB ras-1 (κB-ras-1) and kappaB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with IκB (IκB), an inhibitor of the transcription factor NF-κB. IκB exists in two homologous forms, IκB-alpha and IκB-beta, although IκB-beta contains a unique 47-amino acid region within its ankyrin domain. While inactive IκB-alpha-NF-κB complexes can shuttle in and out of the nucleus, IκB-beta-NF-κB complexes are retained exclusively in the cytoplasm. It is suggested that kappaB-ras proteins preferentially bind to the IκB-beta form through this unique insert within the ankyrin region, thus modulating the cellular location of IκB-beta and regulating the rate of degradation of IκB-beta. This antibody detects both kappaB-ras1 and kappaB-ras2. |
| REFERENCES: | 1) Fenwick C, Na SY, Voll RE, et al. A subclass of Ras proteins that regulate the degradation of IkappaB. Science 2000; 287:869-73. | 2) Chen Y, Wu J and Ghosh G. KappaB-Ras binds to the unique insert within the ankyrin repeat domain if IkappaBbeta and regulates cytoplasmic retention of IkappaBbeta x NF-kappaB complexes. J. Biol. Chem.2003; 278:23101-6. |
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