KANK3 Antibody Cat. No.: 7311

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
IMMUNOGEN: KANK3 antibody was raised against an 18 amino acid peptide near the amino terminus of human KANK3 .

The immunogen is located within amino acids 50 - 100 of KANK3.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: KANK3 Antibody can be used for detection of KANK3 by Western blot starting at 1 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
SPECIFICITY: Two alternatively spliced transcript variants encoding different isoforms have been identified.
POSITIVE CONTROL:1) Cat. No. 1202 - A431 Cell Lysate
 2) Cat. No. 10-401 - Human Kidney Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 90 kDa

Observed: 100 kDa

psi-iconProperties
PURIFICATION:KANK3 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:KANK3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KANK3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:KANK3
ALTERNATE NAMES:KANK3 Antibody: ANKRD47, ANKRD47, KN motif and ankyrin repeat domain-containing protein 3, Ankyrin repeat domain-containing protein 47
ACCESSION NO.:NP_940873
PROTEIN GI NO.:157504499
GENE ID:256949
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:KANK3 Antibody: Ankyrins are membrane adaptor molecules that play important roles in the control of cytoskeleton formation by regulating actin polymerization. Like other members of the KANK family, KANK3 (KN motif and ankyrin repeat domain-containing protein 3), is thought to play a role in the formation of actin stress fibers. In zebrafish, the homolog of KANK3 interacts with the adaptor protein Numb, a protein implicated in multiple basic cellular processes, and is essential for epidermal integrity and neurulation, suggesting that KANK3 may play a similar role in higher organisms.
REFERENCES:1) Zhu Y, Kakinuma N, Wang Y, et al. Kank proteins: a new family of ankyrin-repeat domain containing proteins. Biochim. Biophys. Acta 2008; 1780:128-33.
2) Roy BC, Kakinuma N, Kiyama R. Kank attenuates actin remodeling by preventing interaction between IRSp53 and Rac1. J. Cell Biol. 2009; 184:253-67.
3) Boggetti B, Jasik J, Takamiya M, et al. NBP, a zebrafish homolog of human Kank3, is a novel Numb interactor essential for epidermal integrity and neurulation. Dev. Biol. 2012; 365:164-74.

ANTIBODIES FOR RESEARCH USE ONLY.

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