KANK2 Antibody Cat. No.: 7309

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (94%)
IMMUNOGEN: KANK2 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human KANK2 .

The immunogen is located within the last 50 amino acids of KANK2.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: KANK2 Antibody can be used for detection of KANK2 by Western blot at 1 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: Two alternatively spliced transcript variants encoding different isoforms have been identified.
POSITIVE CONTROL:1) Cat. No. 1403 - Mouse Brain Tissue Lysate
 2) Cat. No. 10-401 - Human Kidney Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 95 kDa

Observed: 102 kDa

psi-iconProperties
PURIFICATION:KANK2 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:KANK2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KANK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:KANK2
ALTERNATE NAMES:KANK2 Antibody: SIP, MXRA3, ANKRD25, KIAA1518, SIP, KN motif and ankyrin repeat domain-containing protein 2, Ankyrin repeat domain-containing protein 25
ACCESSION NO.:NP_056308
PROTEIN GI NO.:258613875
GENE ID:25959
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:KANK2 Antibody: Ankyrins are membrane adaptor molecules that play important roles in the control of cytoskeleton formation by regulating actin polymerization. KANK2 (KN motif and ankyrin repeat domain-containing protein 2), was initially identified as a steroid receptor coactivator (SRC) interacting protein (SIP) that could sequester steroid receptor coactivators in the cytoplasm. More recent experiments have shown that while KANK2 is widely expressed, in kidney podocytes, KANK2 localizes to foot processes, suggesting that KANK2 may contribute to controlling actin dynamics in podocyte foot processes.
REFERENCES:1) Zhu Y, Kakinuma N, Wang Y, et al. Kank proteins: a new family of ankyrin-repeat domain containing proteins. Biochim. Biophys. Acta 2008; 1780:128-33.
2) Roy BC, Kakinuma N, Kiyama R. Kank attenuates actin remodeling by preventing interaction between IRSp53 and Rac1. J. Cell Biol. 2009; 184:253-67.
3) Zhang Y, Zhang H, Liang J, et al. SIP, a novel ankyrin repeat containing protein, sequesters steroid receptor coactivators in the cytoplasm. EMBO J. 2007; 26:2645-57.
4) Xu X, Patrakka J, Sistani L, et al. Expression of novel podocyte-associated proteins sult1b1 and ankrd25. Nephron Exp. Nephrol. 2011; 117:e39-46.

ANTIBODIES FOR RESEARCH USE ONLY.

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