Datasheet

KANK2 Antibody
CATALOG NUMBER: 7309

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (94%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:KANK2 Antibody can be used for detection of KANK2 by Western blot at 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1403 - Mouse Brain Tissue Lysate
 2) Cat. No. 10-401 - Human Kidney Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 95 kDa

Observed: 102 kDa
SPECIFICITY:Two alternatively spliced transcript variants encoding different isoforms have been identified.
IMMUNOGEN:KANK2 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human KANK2 .

The immunogen is located within the last 50 amino acids of KANK2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:KANK2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:KANK2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KANK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:KANK2 Antibody: SIP, MXRA3, ANKRD25, KIAA1518, SIP, KN motif and ankyrin repeat domain-containing protein 2, Ankyrin repeat domain-containing protein 25
ACCESSION NO.:NP_056308
PROTEIN GI NO.:258613875
OFFICIAL SYMBOL:KANK2
GENE ID:25959

Background

BACKGROUND:KANK2 Antibody: Ankyrins are membrane adaptor molecules that play important roles in the control of cytoskeleton formation by regulating actin polymerization. KANK2 (KN motif and ankyrin repeat domain-containing protein 2), was initially identified as a steroid receptor coactivator (SRC) interacting protein (SIP) that could sequester steroid receptor coactivators in the cytoplasm. More recent experiments have shown that while KANK2 is widely expressed, in kidney podocytes, KANK2 localizes to foot processes, suggesting that KANK2 may contribute to controlling actin dynamics in podocyte foot processes.
REFERENCES: 1) Zhu Y, Kakinuma N, Wang Y, et al. Kank proteins: a new family of ankyrin-repeat domain containing proteins. Biochim. Biophys. Acta 2008; 1780:128-33.
2) Roy BC, Kakinuma N, Kiyama R. Kank attenuates actin remodeling by preventing interaction between IRSp53 and Rac1. J. Cell Biol. 2009; 184:253-67.
3) Zhang Y, Zhang H, Liang J, et al. SIP, a novel ankyrin repeat containing protein, sequesters steroid receptor coactivators in the cytoplasm. EMBO J. 2007; 26:2645-57.
4) Xu X, Patrakka J, Sistani L, et al. Expression of novel podocyte-associated proteins sult1b1 and ankrd25. Nephron Exp. Nephrol. 2011; 117:e39-46.

For Research Use Only