Datasheet

KANK1 Antibody
CATALOG NUMBER: 7307

Availability: In stock

$0.00
Product Price Options

* Required Fields

$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:KANK1 Antibody can be used for detection of KANK1 by Western blot at 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
 2) Cat. No. 10-401 - Human Kidney Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 149 kDa

Observed: 150 kDa
SPECIFICITY:Two alternatively spliced transcript variants encoding different isoforms have been identified. The lower molecular weight band seen in the immunoblot is thought to be non-specific.
IMMUNOGEN:KANK1 antibody was raised against a 19 amino acid peptide near the amino terminus of human KANK1 .

The immunogen is located within the first 50 amino acids of KANK1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:KANK1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:KANK1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:KANK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:KANK1 Antibody: KANK, CPSQ2, ANKRD15, KANK, KIAA0172, KN motif and ankyrin repeat domain-containing protein 1, Ankyrin repeat domain-containing protein 15
ACCESSION NO.:NP_055973
PROTEIN GI NO.:64464726
OFFICIAL SYMBOL:KANK1
GENE ID:23189

Background

BACKGROUND:KANK1 Antibody: Ankyrins are membrane adaptor molecules that play important roles in the control of cytoskeleton formation by regulating actin polymerization. KANK1 (KN motif and ankyrin repeat domain-containing protein 1), also known as ANKRD15, is a 1,352 amino acid protein that contains at least 12 exons and 5 ANK repeats. It binds to beta-catenin and regulates its subcellular distribution. KANK1 is ubiquitously expressed and localizes to cytoplasm. It may function as a tumor suppressor for renal cell carcinoma. Mutations in this gene cause cerebral palsy spastic quadriplegic type 2, a central nervous system development disorder.
REFERENCES: 1) Zhu Y, Kakinuma N, Wang Y, et al. Kank proteins: a new family of ankyrin-repeat domain containing proteins. Biochim. Biophys. Acta 2008; 1780:128-33.
2) Roy BC, Kakinuma N, Kiyama R. Kank attenuates actin remodeling by preventing interaction between IRSp53 and Rac1. J. Cell Biol. 2009; 184:253-67.
3) Sarkar S, Roy BC, Hatano N, et al. A novel ankyrin repeat-containing gene (Kank) located at 9p24 is a growth suppressor of renal cell carcinoma. J. Biol. Chem. 2002; 277:36585-91.
4) Lerer I, Sagi M, Meiner V, et al. Deletion of the ANKRD15 gene at 9p24.3 causes parent-of-origin-dependent inheritance of familial cerebral palsy. Hum. Mol. Genet. 2005; 14: 3911-20

For Research Use Only