JMJD7 Antibody Cat. No.: 5385

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: JMJD7 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human JMJD7.

The immunogen is located within amino acids 230 - 280 of JMJD7.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: JMJD7 antibody can be used for detection of JMJD7 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate

psi-iconProperties
PURIFICATION:JMJD7 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:JMJD7 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:JMJD7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:JMJD7
ALTERNATE NAMES:JMJD7 Antibody: JmjC domain-containing protein 7, Jumonji domain-containing protein 7
ACCESSION NO.:P0C870
PROTEIN GI NO.:205783894
GENE ID:100137047
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:JMJD7 Antibody: The jumonji domain-containing protein (JMJD) family is defined by the presence of the JmjC domain that is observed in several diverse species. While several JMJD proteins have been identified as being involved in chromatin regulation, histone demethylation and development, the function of JMJD7 has not been identified. JMJD7 was initially thought to be a novel splice form of the phospholipase PLA2G4B which is located downstream of JMJD7 as most tissues express a read-through transcript of the two genes.
REFERENCES:1) Takeuchi T, Watanabe Y, Takano-Shimizu T, et al. Roles of jumonji and jumonji family genes in chromatin regulation and development. Dev. Dyn.2006; 235:2449-59.
2) Clissold PM and Ponting CP. JmjC: cupin metalloenzyme-like domains in jumonji, hairless and phospholipase A2beta. Trends Biochem.Sci. 2001; 26:7-9.

ANTIBODIES FOR RESEARCH USE ONLY.

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