Datasheet

JMJD3 Antibody
CATALOG NUMBER: 6657

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:JMJD3 antibody can be used for detection of EPAC1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-004 - K-562 Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 167, 181 kDa

Observed: 180 kDa
SPECIFICITY:This antibody is specific for JMJD3 and will not recognize other JMJD proteins.
IMMUNOGEN:JMJD3 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human JMJD3.

The immunogen is located within amino acids 1370 - 1420 of JMJD3.
HOST SPECIES:Rabbit

Properties

PURIFICATION:JMJD3 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:JMJD3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:JMJD3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:JMJD3 Antibody: JMJD3, JMJD3, KIAA0346, Lysine-specific demethylase 6B, JmjC domain-containing protein 3
ACCESSION NO.:EAW90126
PROTEIN GI NO.:122937251
OFFICIAL SYMBOL:KDM6B
GENE ID:23135

Background

BACKGROUND:JMJD3 Antibody: The Jumonji domain-containing protein 3 (JMJD3) functions as a trimethylation-specific demethylase, converting the trimethylated histone H3 Lys27 residue to the dimethylated form, and is thought to also function as a transcriptional repressor. JMJD3 plays a central role in regulation of posterior development, by regulating HOX gene expression. It is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. JMJD3 can also interact with and demethylate p53, resulting in its stabilization and localization to the nucleus in mouse embryo fibroblasts during neural stem cell differentiation.
REFERENCES: 1) Agger K, Cloos PA, Christensen J, et al. UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene regulation and development. Nature 2007; 449:731-4.
2) De Santa F, Totaro MG, Prosperini E, et al. The histone H3 lysine-27 demethylase Jmjd3 links inflammation to inhibition of polycomb-mediated silencing. Cell 2007; 130:1083-94
3) Sola S, Xavier JM, Santos DM, et al. p53 interaction with JMJD3 results in its nuclear distribution during mouse neural stem cell differentiation. PLoS One 2011; 6:e18421.

For Research Use Only