Datasheet

JMJD2A Antibody
CATALOG NUMBER: 5373

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:JMJD2A antibody can be used for detection of JMJD2A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1464 - Rat Liver Tissue Lysate
IMMUNOGEN:JMJD2A antibody was raised against a 14 amino acid synthetic peptide from near the center of human JMJD2A.

The immunogen is located within amino acids 340 - 390 of JMJD2A.
HOST SPECIES:Rabbit

Properties

PURIFICATION:JMJD2A Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:JMJD2A Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:JMJD2A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:JMJD2A Antibody: JMJD2, JHDM3A, JMJD2A, TDRD14A, JMJD2, KIAA0677, Lysine-specific demethylase 4A, JmjC domain-containing histone demethylation protein 3A
ACCESSION NO.:CAH71021
PROTEIN GI NO.:55665236
OFFICIAL SYMBOL:KDM4A
GENE ID:9682

Background

BACKGROUND:JMJD2A Antibody: Members of the Jumonji domain 2 (JMJD2) family contain a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. The first member of this group, JMJD2A, is widely expressed in human tissues and cell lines and functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. JMJD2A can also form a complex with the androgen receptor (AR), a transcription factor that is pivotal for the development of prostate cancer. Overexpression of JMJD2A stimulates AR function and this stimulation is dependent on JMJD2A catalytic activity, suggesting that JMJD2A might be a critical protein with roles in cell proliferation and oncogenesis.
REFERENCES: 1) Gray SG, Iglesias AH, Lizcano F, et al. Functional characterization of JMJD2A, a histone deacetylase- and retinoblastoma-binding protein. J. Biol. Chem.2005; 31:28507-18.
2) Shin S and Janknecht R. Activation of androgen receptor by histone demethylases JMJD2A and JMJD2D. Biochem. Biophys. Res. Comm.2007; 359:742-6.

For Research Use Only