Datasheet

JMJD1C Antibody
CATALOG NUMBER: 5371

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:JMJD1C antibody can be used for detection of JMJD1C by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1304 - Human Liver Tissue Lysate
SPECIFICITY:This antibody will not cross-react with JMJD1A or JMJD1B.
IMMUNOGEN:JMJD1C antibody was raised against a 20 amino acid synthetic peptide from near the amino terminus of human JMJD1C.

The immunogen is located within amino acids 380 - 430 of JMJD1C.
HOST SPECIES:Rabbit

Properties

PURIFICATION:JMJD1C Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:JMJD1C Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:JMJD1C antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:JMJD1C Antibody: TRIP8, JHDM2C, KIAA1380, TRIP8, Probable JmjC domain-containing histone demethylation protein 2C, Jumonji domain-containing protein 1C, TR-interacting protein 8
ACCESSION NO.:NP_116165
PROTEIN GI NO.:118600981
OFFICIAL SYMBOL:JMJD1C
GENE ID:221037

Background

BACKGROUND:JMJD1C Antibody: The jumonji domain containing 1C protein (JMJD1C) was initially discovered in silico, and later suggested to be a candidate gene for autism. Like the related proteins JMJD1A and JMJD1B, JMJD1C is a histone H3K9 demethylase implicated in the nuclear hormone receptor-based transcriptional regulation. JMJD1C mRNA is highly expressed in undifferentiated embryonic stem (ES) cells as well as pancreatic islet, diffuse-type gastric cancer, and other tissues and tumors. The JMJD1C gene promoter contain bHLH-, AP-1-, and POU5F1-binding sites, and as preferential expression of POU5F1 has been reported in ES cells, pancreatic islet, and diffuse-type gastric cancer, it has been suggested that POU5F1-mediated expression of JMJD1C reactivates previously silenced genes in ES cells and diffuse-type gastric cancer. At least three isoforms of JMJD1C are known to exist.
REFERENCES: 1) Katoh M and Katoh M. Identification of TRIP8 gene in silico. Int. J. Mol. Med.2003; 12:817-21.
2) Castermans D, Vermeesch JR, Fryns JP, et al. Identification and characterization of the TRIP8 and REEP3 genes on chromosome 10q21.3 as novel candidate genes for autism. Eur. J. Hum. Genet.2007; 15:422-31.
3) Katoh M and Katoh M. Comparative integromics on JMJD1C gene encoding histone demethylase: Conserved POU5F1 binding site elucidating mechanism of JMJD1C expression in undifferentiated ES cells and diffuse-type gastric cancer. Int. J. Oncology2007; 31:219-23.
4) Katoh Y and Katoh M. Conserved POU-binding site linked to SP1-binding site within FZD5 promoter: transcriptional mechanism of FZD5 in undifferentiated human ES cells, fetal liver/spleen, adult colon, pancreatic islet, and diffuse-type gastric cancer. Int. J. Oncol.2007; 30:751-5.

For Research Use Only