Datasheet

JMJD1B Antibody
CATALOG NUMBER: 5369

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:JMJD1B antibody can be used for detection of JMJD1B by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1464 - Rat Liver Tissue Lysate
SPECIFICITY:This antibody will not cross-react with JMJD1A or JMJD1C.
IMMUNOGEN:JMJD1B antibody was raised against a 20 amino acid synthetic peptide from near the center of human JMJD1B.

The immunogen is located within amino acids 1050 - 1100 of JMJD1B.
HOST SPECIES:Rabbit

Properties

PURIFICATION:JMJD1B Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:JMJD1B Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:JMJD1B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:JMJD1B Antibody: 5qNCA, NET22, C5orf7, JMJD1B, JHDM2B, KIAA1082, Lysine-specific demethylase 3B, JmjC domain-containing histone demethylation protein 2B
ACCESSION NO.:EAW62141
PROTEIN GI NO.:119582545
OFFICIAL SYMBOL:KDM3B
GENE ID:51780

Background

BACKGROUND:JMJD1B Antibody: The jumonji domain containing 1B protein (JMJD1B) was originally discovered as a candidate for the myeloid leukemia tumor suppressor gene. Besides CD34+ cells and acute myeloid leukemia (AML) cell lines, JMJD1B mRNA is highly expressed in liver, heart, kidney, skeletal muscle, and placenta tissues. The JMJD1B gene is localized to a region of chromosome 5q31, which is frequently deleted in myeloid leukemias and myelodysplasias and expression of JMJD1B in a del(5q) cell line results in suppression of clonogenic growth suggesting that JMJD1B may function as a tumor suppressor. In contrast, JMJD1B gene copy number and mRNA expression level was increased in several non-small cell lung cancers indicating that the role of JMJD1B in cancer formation and progression is more complex than originally postulated. At least three isoforms of JMJD1B are known to exist.
REFERENCES: 1) Lai F, Godley LA, Fernald AA, et al. cDNA cloning and genomic structure of three genes localized to human chromosome band 5q31 encoding potential nuclear proteins. Genomics2000; 70:123-30.
2) Hu Z, Gomes I, Horrigan SK, et al. A novel nuclear protein, 5qNCA (LOC51780) is a candidate for the myeloid leukemia tumor suppressor gene on chromosome band q31. Oncogene2001; 20:6946-54.
3) Baik S-H, Jee B-K, Choi J-S, et al. DNA profiling by array comparative genomic hybridization (CGH) of peripheral blood mononuclear cells (PBMC) and tumor tissue cell in non-small cell lung cancer (NSCLC). Mol. Biol. Rep.2009; 36:1767-78.

For Research Use Only