JMJD1A Antibody Cat. No.: 5365

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (94%), Chicken: (87%)
IMMUNOGEN: Anti-JMJD1A antibody (5365) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human JMJD1A.

The immunogen is located within amino acids 50-100 of JMJD1A.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: WB: 1-2 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples; Immunofluorescence in rat samples. All other applications and species not yet tested.
SPECIFICITY: This antibody will not cross-react with JMJD1B or JMJD1C.
POSITIVE CONTROL:1) Cat. No. 1211 - HepG2 Cell Lysate
 2) Cat. No. 1201 - HeLa Cell Lysate
 3) Cat. No. 1219 - MCF7 Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 147kD

Observed: 147kD
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar JMJD1A expression profile in human cell lines detected by two independent anti-JMJD1A antibodies that recognize different epitopes, 5365 against N-terminus domain and 5367 against central domain.  JMJD1A proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

ISOFORMS: Human JMJD1A has one isoform (1321aa, 147kD). Mouse JMJD1A has 2 isoforms, including isoform 1 (1323aa, 148kD) and isoform 2 (831aa, 94kD). Rat JMJD1A has one isoform (1323aa, 148kD). 5365 can detect human, mouse and rat.

psi-iconProperties
PURIFICATION:JMJD1A Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:JMJD1A Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:JMJD1A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:KDM3A
ALTERNATE NAMES:JMJD1A Antibody: TSGA, JMJD1, JHDM2A, JHMD2A, JMJD1A, KIAA0742, TSGA, Lysine-specific demethylase 3A, JmjC domain-containing histone demethylation protein 2A
ACCESSION NO.:NP_060903
PROTEIN GI NO.:20357522
GENE ID:55818
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:JMJD1A Antibody: The jumonji domain containing 1A protein (JMJD1A) was originally discovered as a testes specific gene, but has been found to be expressed in numerous tissues. JMJD1A is a histone demethylase and specifically demethylates mono- and dimethyl-H3K9. It has also been found to be a novel interaction partner with ER71, a transcription factor expressed in the testes of adult mice and during embryogenesis. JMJD1A is also a downstream gene of STAT3, a protein that has been found to be important in the maintenance of mouse embryonic stem (mES) cells, and decreased JMJD1A expression correlated with the differentiation of cultured mES cells following the removal of the cytokine LIF. These findings suggest that JMJD1A might be a critical signaling molecule underlying the maintenance of pluripotency in embryonic stem cells. At least two isoforms of JMJD1A are known to exist.
REFERENCES:1) Hoog et al. Mol. Reprod. Dev.1991; 30:173-81.
2) Sar et al. Cell Tissue Res.2009; 337:223-34.
3) Yamane et al. Cell. 2006; 125:483-95.
4) Knebel et al. J. Cell Biochem.2006; 99:319-29.

ANTIBODIES FOR RESEARCH USE ONLY.

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