Datasheet

JMJD1A Antibody
CATALOG NUMBER: 5365

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Chicken: (87%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:JMJD1A antibody can be used for detection of JMJD1A by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1211 - HepG2 Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 145 kDa

Observed: 145 kDa
SPECIFICITY:This antibody will not cross-react with JMJD1B or JMJD1C.
IMMUNOGEN:JMJD1A antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human JMJD1A.

The immunogen is located within amino acids 50 - 100 of JMJD1A.
HOST SPECIES:Rabbit

Properties

PURIFICATION:JMJD1A Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:JMJD1A Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:JMJD1A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:JMJD1A Antibody: TSGA, JMJD1, JHDM2A, JHMD2A, JMJD1A, KIAA0742, TSGA, Lysine-specific demethylase 3A, JmjC domain-containing histone demethylation protein 2A
ACCESSION NO.:NP_060903
PROTEIN GI NO.:20357522
OFFICIAL SYMBOL:KDM3A
GENE ID:55818

Background

BACKGROUND:JMJD1A Antibody: The jumonji domain containing 1A protein (JMJD1A) was originally discovered as a testes specific gene, but has been found to be expressed in numerous tissues. JMJD1A is a histone demethylase and specifically demethylates mono- and dimethyl-H3K9. It has also been found to be a novel interaction partner with ER71, a transcription factor expressed in the testes of adult mice and during embryogenesis. JMJD1A is also a downstream gene of STAT3, a protein that has been found to be important in the maintenance of mouse embryonic stem (mES) cells, and decreased JMJD1A expression correlated with the differentiation of cultured mES cells following the removal of the cytokine LIF. These findings suggest that JMJD1A might be a critical signaling molecule underlying the maintenance of pluripotency in embryonic stem cells. At least two isoforms of JMJD1A are known to exist.
REFERENCES: 1) Hoog C, Schalling M, Grunder-Brundell E, et al. Analysis of a murine male germ cell-specific transcript that encodes a putative zinc finger protein. Mol. Reprod. Dev.1991; 30:173-81.
2) Sar A, Ponjevic D, Nguyen M, et al. Identification and characterization of demethylase JMJD1A as a gene upregulated in the human cellular response to hypoxia. Cell Tissue Res.2009; 337:223-34.
3) Yamane K, Toumazou C, Tsukada Y, et al. JHDM2A, a JmjC-containing H3K9 demethylase, facilitates transcription activation by androgen receptor. Cell2006; 125:483-95.
4) Knebel J, De Haro L, and Janknecht R. Repression of transcription by TSGA/Jmjd1a, a novel interaction partner of the ETS protein ER71. J. Cell Biochem.2006; 99:319-29.

For Research Use Only