Datasheet

JAK2 Antibody
CATALOG NUMBER: 8085

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (82%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:JAK2 antibody can be used for detection of JAK2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1212 - 3T3 Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 125 kDa

Observed: 125 kDa
SPECIFICITY:JAK2 antibody is human, mouse and rat reactive. JAK2 antibody is predicted to not cross-react with other members of the JAK family of proteins.
IMMUNOGEN:JAK2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human JAK2.

The immunogen is located within the last 50 amino acids of JAK2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:JAK2 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:JAK2 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:JAK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Janus kinase 2, JTK10, THCYT3
ACCESSION NO.:NP_004963
PROTEIN GI NO.:4826776
OFFICIAL SYMBOL:JAK2
GENE ID:3717

Background

BACKGROUND:JAK2 is a member of the JAK family of kinases that also include JAK1, JAK3, and TYK2 (1,2). JAK kinases are activated following tyrosine phosphorylation of cytokine receptors after ligand binding. JAK2 activation promotes the recruitment and phosphorylation of STAT3 and STAT5. These transcription factors then translocate to the nucleus where they bind specific DNA promoter sequences resulting in the transcription of genes that regulate cell proliferation, differentiation, and apoptosis (3). Mice with a disrupted JAK2 gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis (4).
REFERENCES: 1) Wilks AF, Harpur AG, Kurban RR, et al. Two novel protein-tyrosine kinases, each with a second phosphotransferase-related catalytic domain, define a new class of protein kinase. Mol. Cell Biol. 1991; 11:2057-66.
2) Quintas-Cardama A and Verstovsek S. Molecular pathways: JAK/STAT pathway: mutations, inhibitors, and resistance. Clin. Can. Res. 2013; 19:1933-40.
3) Levine RL, Pardanani A, Tefferi A, et al. Role of JAK2 in the pathogenesis and therapy of myeloproliferative disorders. Nat. Rev. Cancer 2007; 7:673-83.
4) Neubauer H, Cumano A, Muller M, et al. Jak2 deficiency defines an essential developmental checkpoint in definitive hematopoiesis. Cell 1998; 93:397-409.

For Research Use Only