Datasheet

JAGN1 Antibody
CATALOG NUMBER: 8367

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (95%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:JAGN1 antibody can be used for detection of JAGN1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1411 - Mouse Colon Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 20 kDa

Observed: 25 kDa
SPECIFICITY:JAGN1 antibody is human, mouse and rat reactive. At least four isoforms of JAGN1 are known to exist; this antibody will detect all but isoform 2.
IMMUNOGEN:JAGN1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human JAGN1.

The immunogen is located within the last 50 amino acids of JAGN1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:JAGN1 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:JAGN1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:JAGN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Jagunal homolog 1
ACCESSION NO.:NP_115881
PROTEIN GI NO.:190014601
OFFICIAL SYMBOL:JAGN1
GENE ID:84522

Background

BACKGROUND:JAGN1 is the human ortholog of a gene originally identified in Drosophila melanogaster. It is a transmembrane protein that functions in the early secretory pathway and is necessary for neutrophil differentiation and survival. Individuals with mutations in the JAGN1 gene causes aberrant myeloid cell homeostasis and severe congenital neutropenia (SCN) (1). JAGN1-mutant granulocytes are characterized by ultrastructural defects, aberrant N-glycosylation of multiple proteins, and increased apoptosis (1). Mice lacking this gene could not mount and efficient neutrophil-dependent immune response to a human fungal pathogen (2).
REFERENCES: 1) Boztug K, Jarvinen PM, Salzer E, et al. JAGN1 deficiency causes aberrant myeloid cell homeostasis and congenital neutropenia. Nat. Genet. 2014; 46:1021-7.
2) Wirnsberger G, Zwolanek F, Stadlmann J, et al. Jagunal homolog 1 is a critical regulator of neutrophil function in fungal host defense. Nat. Genet. 2014; 46:1028-33.

For Research Use Only