Datasheet

JAG1 Antibody
CATALOG NUMBER: 8059

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:JAG1 antibody can be used for detection of JAG1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 10-901 - Human Spleen Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 134 kDa

Observed: 130 kDa
SPECIFICITY:JAG1 antibody is human, mouse and rat reactive. At least two isoforms of JAG1 are known to exist; this antibody will detect both isoforms.
IMMUNOGEN:JAG1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human JAG1.

The immunogen is located within the last 50 amino acids of JAG1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:JAG1 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:JAG1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:JAG1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Jagged 1, AGS, AHD, AWS, CD339, HJ1, JAGL1
ACCESSION NO.:NP_000205
PROTEIN GI NO.:4557679
OFFICIAL SYMBOL:JAG1
GENE ID:182

Background

BACKGROUND:The LIN-12/Notch family of transmembrane receptors plays a central role in development by regulating cell fate decisions (1). Ligands for Notch include JAG1, JAG2 and Delta. JAG1 is one of the DSL (Delta, Serrate, Lag2) transmembrane proteins that can activate Notch. It is involved in mammalian cardiovascular development and in cell-fate decisions during hematopoiesis (1,2). JAG1 is widely expressed in adult and fetal tissues and the mutation of the JAG1 gene is associated with Familial Tetrology of Fallot (3). JAG1 expression is associated with prostate cancer metastasis and is up-regulated in cervical squamous cell carcinoma (4,5).
REFERENCES: 1) Lai EC. Notch signaling: control of cell communication and cell fate. Development 2004; 131:965-73.
2) Milner LA, Kopan R, Martin DI, et al. A human homologue of the Drosophila developmental gene, Notch, is expressed in CD34+ hematopoietic precursors. Blood1994; 83:2057-62.
3) Guida V, Chiappe F, Ferese R, et al. Novel and recurrent JAG1 mutations in patients with tetralogy of Fallot. Clin. Genet. 2011; 80:591-4.
4) Santagata S, Demichelis F, Riva A, et al. JAGGED1 expression is associated with prostate cancer metastasis and recurrence. Cancer Res. 2004; 64:6854-7.

For Research Use Only