IZUMO1 Antibody Cat. No.: 8233

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (82%), Mouse: (81%)
IMMUNOGEN: IZUMO1 antibody was raised against an 18 amino acid peptide near the center of human IZUMO1.

The immunogen is located within amino acids 170 - 220 of IZUMO1.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: IZUMO1 antibody can be used for detection of IZUMO1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
SPECIFICITY: IZUMO1 antibody is human specific. At least four isoforms of IZUMO1 are known to exist; this antibody will detect the two longest isoforms.
POSITIVE CONTROL:1) Cat. No. 1313 - Human Testis Tissue Lysate
 2) Cat. No. 11-701 - Human Testis Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 35, 39, 42 kDa

Observed: 52 kDa

psi-iconProperties
PURIFICATION:IZUMO1 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:IZUMO1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IZUMO1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:IZUMO1
ALTERNATE NAMES:Izumo sperm-egg fusion 1, IZUMO, Izumo sperm-egg fusion protein 1, Oocyte binding/fusion factor, OBF, Sperm-specific protein izumo
ACCESSION NO.:NP_872381
PROTEIN GI NO.:194097475
GENE ID:284359
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:IZUMO1, a sperm-specific protein, is essential for sperm-egg plasma membrane binding and fusion. It is involved in the fertilization process and in the fusion of the sperm with the egg (1,2). IZUMO1 is expressed as at least four isoforms produced by alternative splicing and contains one Ig-like (immunoglobulin-like) C2-type domain (3). Lack of IZUMO1 in males, due to homozygous mutation in the gene that encodes IZUMO1, results in sterility due to an inability to penetrate the plasma membrane of the egg (4).
REFERENCES:1) Inoue N, Hamada D, Kamikubo H, et al. Molecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion. Development 2013; 140:3221-9.
2) Inoue N, Ikawa M, and Okabe M. The mechanism of sperm-egg interaction and the involvement of IZUMO1 in fusion. Asian J. Androl. 2011; 13:81-7.
3) Xing WJ, Han BD, Wu Q, et al. Molecular cloning and characterization of Izumo1 gene from sheep and cashmere goat reveal alternative splicing. Mol. Biol. Rep. 2011; 38:1995-2006.
4) Inoue N, Ikawa M, Isotani A, et al. The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs. Nature 2005; 434:234-8.

ANTIBODIES FOR RESEARCH USE ONLY.

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