Datasheet

IZUMO1 Antibody
CATALOG NUMBER: 8233

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rat: (82%), Mouse: (81%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:IZUMO1 antibody can be used for detection of IZUMO1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1313 - Human Testis Tissue Lysate
 2) Cat. No. 11-701 - Human Testis Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 35, 39, 42 kDa

Observed: 52 kDa
SPECIFICITY:IZUMO1 antibody is human specific. At least four isoforms of IZUMO1 are known to exist; this antibody will detect the two longest isoforms.
IMMUNOGEN:IZUMO1 antibody was raised against an 18 amino acid peptide near the center of human IZUMO1.

The immunogen is located within amino acids 170 - 220 of IZUMO1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IZUMO1 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IZUMO1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IZUMO1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Izumo sperm-egg fusion 1, IZUMO, Izumo sperm-egg fusion protein 1, Oocyte binding/fusion factor, OBF, Sperm-specific protein izumo
ACCESSION NO.:NP_872381
PROTEIN GI NO.:194097475
OFFICIAL SYMBOL:IZUMO1
GENE ID:284359

Background

BACKGROUND:IZUMO1, a sperm-specific protein, is essential for sperm-egg plasma membrane binding and fusion. It is involved in the fertilization process and in the fusion of the sperm with the egg (1,2). IZUMO1 is expressed as at least four isoforms produced by alternative splicing and contains one Ig-like (immunoglobulin-like) C2-type domain (3). Lack of IZUMO1 in males, due to homozygous mutation in the gene that encodes IZUMO1, results in sterility due to an inability to penetrate the plasma membrane of the egg (4).
REFERENCES: 1) Inoue N, Hamada D, Kamikubo H, et al. Molecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion. Development 2013; 140:3221-9.
2) Inoue N, Ikawa M, and Okabe M. The mechanism of sperm-egg interaction and the involvement of IZUMO1 in fusion. Asian J. Androl. 2011; 13:81-7.
3) Xing WJ, Han BD, Wu Q, et al. Molecular cloning and characterization of Izumo1 gene from sheep and cashmere goat reveal alternative splicing. Mol. Biol. Rep. 2011; 38:1995-2006.
4) Inoue N, Ikawa M, Isotani A, et al. The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs. Nature 2005; 434:234-8.

For Research Use Only