| SPECIES: | Human, Mouse, Rat |
| SOURCE SPECIES: | Human Cells |
| SEQUENCE: | Asp32-Glu143 |
| FUSION TAG: | C-6 His tag |
| TESTED APPLICATIONS: | |
| APPLICATIONS: | This recombinant protein can be used for biological assays. For research use only. |
Specifications| PURITY: | Greater than 95% as determined by reducing SDS-PAGE. Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
| PREDICTED MOLECULAR WEIGHT: | 13.6 kD |
| PHYSICAL STATE: | Lyophilized |
| BUFFER: | Lyophilized from a 0.2 um filtered solution of PBS, pH7.4. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
| STORAGE CONDITIONS: | Lyophilized protein should be stored at -20˚C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7˚C for 2-7 days. Aliquots of reconstituted samples are stable at -20˚C for 3 months. |
| ALTERNATE NAMES: | Fibronectin type III domain-containing protein 5, Fibronectin type III repeat-containing protein 2, Irisin, FNDC5 |
| ACCESSION NO.: | Q8NAU1 |
| PROTEIN GI NO.: | 929654682 |
| OFFICIAL SYMBOL: | FNDC5 |
| GENE ID: | 252995 |
| BACKGROUND: | Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene.Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation.Mature human, mouse share 100% sequence identity.Irisin induces expression of peroxisome proliferatoractivated receptor gamma coactivator 1 alpha (PGC1 alpha) and uncoupling protein1(UCP1), mitochondrialassociated metabolic proteins. Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue.Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts. |
FOR RESEARCH USE ONLY.
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