Datasheet

IRGC Antibody
CATALOG NUMBER: 5295

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:IRGC antibody can be used for detection of IRGC by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1403 - Mouse Brain Tissue Lysate
 2) Cat. No. 17-208 - A-20 Cell Slide
SPECIFICITY:Two isoforms of IRGC are known to exist; this antibody will recognize both isoforms.
IMMUNOGEN:IRGC antibody was raised against a 12 amino acid synthetic peptide near the carboxy terminus of human IRGC.

The immunogen is located within the last 50 amino acids of IRGC.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IRGC Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IRGC Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IRGC antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IRGC Antibody: IFGGE, IRGC1, Iigp5, CINEMA, R30953_1, IIGP5, Interferon-inducible GTPase 5, Immunity-related GTPase cinema 1
ACCESSION NO.:NP_062558
PROTEIN GI NO.:10257429
OFFICIAL SYMBOL:IRGC
GENE ID:56269

Background

BACKGROUND:IRGC Antibody: Immunity-related GTPases (IRG) (also known as p47 GTPases) are a family of GTPase proteins found in vertebrates, which play critical roles in mediating innate resistance to intracellular pathogens. IRG genes have been found in a number of mammals and lower species including mice, rats, zebrafish and humans. Most of the mouse genes contain interferon-stimulated response elements which mediate transcriptional activation by IFNs. In humans, only two IRG genes have been found: human IRGC encodes a full-length IRG protein that, like the mouse homologue, is constitutively expressed in testis, while human IRGM encodes a considerably truncated protein that is constitutively expressed in cultured cells including some macrophage cell lines. As the two human genes IRGC and IRGM are not subject to IFN control, it has been suggested that the host resistance mechanism supported by IRG proteins in the mouse is lacking in humans.
REFERENCES: 1) Taylor GA. IRG proteins: key mediators of interferon-regulated host resistance to intracellular pathogens. Cell Microbiol.2007; 9:1099-107.
2) Yamada K, Akimoto H, Ogawa Y, et al. Upregulation of immunity-related GTPase (IRG) proteins by TNF-alpha in murine astrocytes. Biochem. Biophys. Res. Commun.2009; 382:434-9.
3) Singh SB, et al. Human IRGM induces autophagy to eliminate intracellular mycobacteria. Science2006; 313:1438-41.
4) Bekpen C, Hunn JP, Rohde C, et al. The interferon-inducible p47 (IRG) GTPases in vertebrates: loss of the cell autonomous resistance mechanism in the human lineage. Genome Biol.2005; 6:R92.

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