| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| IMMUNOGEN: | Anti-IRF7 antibody (8991) was raised against a peptide corresponding to 17 amino acids near the center of human IRF7. The immunogen is located within amino acids 200-230 of IRF7. |
| TESTED APPLICATIONS: | ELISA, IHC-P, WB |
| APPLICATIONS: | WB: 0.5-1 μg/mL; IF: 20 μg/mL; IHC: 2-5 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples. Immunofluorescence in mouse samples. Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested. |
| SPECIFICITY: | Several isoforms of IRF7 are known to exist. |
| POSITIVE CONTROL: | 1) Cat. No. 1203 - A549 Cell Lysate |
| 2) Cat. No. 1201 - HeLa Cell Lysate | |
| 3) Cat. No. 1211 - HepG2 Cell Lysate |
Specifications| VALIDATION: | KO Validation (Figure 1): Anti-IRF7 antibodies (8991) specificity was further verified by IRF7 specific knockout. IRF7 was disrupted in IRF7 knockout A549 cells as compared to that in control wild type cells. |
| ISOFORMS: | Human IRF7 has 4 isoforms, including isoform A (503aa, 54kD), isoform B (474aa, 52kD), isoform C (164aa, 18kD) and isoform D (516aa, 56kD). Mouse IRF7 has one isoform (457aa, 51kD) and Rat IRF7 also has one isoform (456aa, 51kD). 8991 can detect human isoforms and mouse, and possibly can detect rat. |
| PURIFICATION: | IRF7 Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | IRF7 Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | IRF7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | IRF7 |
| ALTERNATE NAMES: | IRF7 Antibody: IRF7A, IRF7B, IRF7C, IRF7H, IRF-7H, Interferon regulatory factor 7, IRF-7 |
| ACCESSION NO.: | Q92985 |
| PROTEIN GI NO.: | 116242593 |
| GENE ID: | 3665 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | IRF7 Antibody: Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF7 play a major role in the innate immune pathway, interacting with the Toll-like receptor (TLR) adaptor proteins MyD88 and Tirp/TRAM and functioning as an intermediate TLR4 and TLR9 signaling. There are at least four differentially spliced isoforms of IRF7, although their function has not been clearly established. |
| REFERENCES: | 1) Malmgaard. J. Interferon & Cyto. Res. 2004; 24:439-54. | 2) Sato et al. Immunity 2000; 13:539-48. | 3) Fitzgerald et al. J. Exp. Med. 2003; 198:1043-55. | 4) Honda et al. Proc. Natl. Acad. Sci. USA 2004; 101:15416-21. |
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