IRF7 Antibody Cat. No.: 8991

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Anti-IRF7 antibody (8991) was raised against a peptide corresponding to 17 amino acids near the center of human IRF7.

The immunogen is located within amino acids 200-230 of IRF7.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: WB: 0.5-1 μg/mL; IF: 20 μg/mL; IHC: 2-5 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples. Immunofluorescence in mouse samples. Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
SPECIFICITY: Several isoforms of IRF7 are known to exist.
POSITIVE CONTROL:1) Cat. No. 1203 - A549 Cell Lysate
 2) Cat. No. 1201 - HeLa Cell Lysate
 3) Cat. No. 1211 - HepG2 Cell Lysate
psi-iconAdvanced Validation
VALIDATION:

KO Validation (Figure 1): Anti-IRF7 antibodies (8991) specificity was further verified by IRF7 specific knockout. IRF7 was disrupted in IRF7 knockout A549 cells as compared to that in control wild type cells.

ISOFORMS: Human IRF7 has 4 isoforms, including isoform A (503aa, 54kD), isoform B (474aa, 52kD), isoform C (164aa, 18kD) and isoform D (516aa, 56kD). Mouse IRF7 has one isoform (457aa, 51kD) and Rat IRF7 also has one isoform (456aa, 51kD). 8991 can detect human isoforms and mouse, and possibly can detect rat.

psi-iconProperties
PURIFICATION:IRF7 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:IRF7 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IRF7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:IRF7
ALTERNATE NAMES:IRF7 Antibody: IRF7A, IRF7B, IRF7C, IRF7H, IRF-7H, Interferon regulatory factor 7, IRF-7
ACCESSION NO.:Q92985
PROTEIN GI NO.:116242593
GENE ID:3665
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:IRF7 Antibody: Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF7 play a major role in the innate immune pathway, interacting with the Toll-like receptor (TLR) adaptor proteins MyD88 and Tirp/TRAM and functioning as an intermediate TLR4 and TLR9 signaling. There are at least four differentially spliced isoforms of IRF7, although their function has not been clearly established.
REFERENCES:1) Malmgaard. J. Interferon & Cyto. Res. 2004; 24:439-54.
2) Sato et al. Immunity 2000; 13:539-48.
3) Fitzgerald et al. J. Exp. Med. 2003; 198:1043-55.
4) Honda et al. Proc. Natl. Acad. Sci. USA 2004; 101:15416-21.

ANTIBODIES FOR RESEARCH USE ONLY.

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