IRF7 Antibody Cat. No.: 8991

Figure 1 KO Validation in A549 Cell Lysate
Loading: 10 μg of A549 WT cell lysates or IRF7 KO cell lysates. Antibodies: IRF7 8991 (0.5 μg/mL) and beta-actin 3779 (1 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. See high quality image.

Figure 2 Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane. Antibodies: IRF7, 8991, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. See high quality image.

Figure 3 Western Blot Validation in Human Tissues
Loading: 15 μg of lysates per lane. Antibodies: IRF7, 8991 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. See high quality image.

Figure 4 Western Blot Validation in Mouse Tissues
Loading: 15 μg of lysates per lane. Antibodies: IRF7, 8991 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. See high quality image.

Figure 6 Immunofluorescence Validation of IRF7 in Mouse Raw264.7 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed Raw264.7 cells labeling IRF7 with 8991 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). See high quality image.

Figure 7 Immunohistochemistry Validation of IRF7 in Human Colon Tissue
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-IRF7 antibody (8991) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. See high quality image.

Figure 8 Immunohistochemistry Validation of IRF7 in Human Pancreas Tissue
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-IRF7 antibody (8991) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. See high quality image.

Figure 9 Immunohistochemistry Validation of IRF7 in Human Liver Tissue
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IRF7 antibody (8991) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. See high quality image.

Figure 10 Immunohistochemistry Validation of IRF7 in Mouse Pancreas Tissue
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-IRF7 antibody (8991) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. See high quality image.

Figure 11 Immunohistochemistry Validation of IRF7 in Rat Brain Tissue
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IRF7 antibody (8991) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. See high quality image.