Datasheet

IRF3 Antibody
CATALOG NUMBER: 3399

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Rat
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:IRF3 antibody can be used for detection of IRF3 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL.

Antibody validated: Western Blot in human and rat samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1305 - Human Kidney Tissue Lysate
 2) Cat. No. 1465 - Rat Kidney Tissue Lysate
 3) Cat. No. 1225 - Ramos Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 50 kDa

Observed: 50 kDa
IMMUNOGEN:IRF3 antibody antibody was raised against a peptide corresponding to 14 amino acids near the center of human IRF3.

The immunogen is located within amino acids 150 - 200 of IRF3.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IRF3 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IRF3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IRF3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IRF3 Antibody: Interferon regulatory factor 3, IRF-3
ACCESSION NO.:NP_001562
PROTEIN GI NO.:4504725
OFFICIAL SYMBOL:IRF3
GENE ID:3661

Background

BACKGROUND:IRF3 Antibody: Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF3 is present in an inactive form in the cytoplasm of most cells. Following viral infection, IRF3 can be activated by IκB kinase-ε and TANK-binding kinase 1 (TBK1), whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA. IRF3 exists as at least two distinct isoforms.
REFERENCES: 1) Malmgaard L. Induction and regulation of IFNs during viral infections. J. Interferon & Cyto. Res. 2004; 24:439-54.
2) Au WC, Moore PA, Lowther W, et al. Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes. Proc. Natl. Acad. Sci. USA 1995; 92:11657-61.
3) Fitzgerald KA, McWhirter SM, Faia KL, et al. IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway. Nat. Immunol. 2003; 4:491-6.
4) Sharma S, Tenoever BR, Grandvaux N, et al. Triggering the interferon antiviral response through an IKK-related pathway. Science 2003; 300:1148-51.

For Research Use Only