IRE1p Antibody Cat. No.: 3655

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Anti-IRE1p antibody (3655) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IRE1P.

The immunogen is located within the last 50 amino acids of IRE1p.
TESTED APPLICATIONS: ELISA, ICC, IF, IHC-P, WB
APPLICATIONS: WB: 0.5-2 μg/mL; ICC: 1 μg/mL; IF: 2-20 μg/mL; IHC: 2 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in mouse samples; Immunofluorescence in human, mouse and rat samples; Immunohistochemistry in human and rat samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1288 - A20 Cell Lysate
 2) Cat. No. 17-208 - A-20 Cell Slide
 3) Cat. No. 1463 - Rat Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 110kD

Observed: 110kD
psi-iconAdvanced Validation
VALIDATION:

KO validation (Figure 2): Anti-IRE1p antibody (3655) specificity was further verified by IRE1P specific knockout. IRE1p signal was disrupted in IRE1p knockout HeLa cells in comparison with that in control HeLa cells.

Induced Expression Validation (Figure 11): IRE1p expression detected by anit-IRE1p antibodies was up-regulated by 20(S)‐PPD treatment.

ISOFORMS: Human IRE1p has 2 isoforms, including isoform 1 (977aa, 110kD) and isoform 2 (70aa, 6.6kD). Mouse IRE1p also has two isoforms, including isoform 1 (977aa, 110kD) and isoform 2 (408aa, 45kD). Rat IRE1p has one isoform (965aa, 109kD). 3655 can detect human, mouse and rat.

psi-iconProperties
PURIFICATION:IRE1p Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:IRE1p Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IRE1p antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:ERN1
ALTERNATE NAMES:IRE1p Antibody: IRE1, IRE1P, IRE1a, hIRE1p, IRE1, Endoplasmic reticulum-to-nucleus signaling 1
ACCESSION NO.:O75460
PROTEIN GI NO.:193806335
GENE ID:2081
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:IRE1p Antibody: Accumulation of malfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94. These proteins are normally bound to ER transmembrane proteins such as IRE1p and ATF6 but ER stress causes their dissociation. This allows IRE1p, a serine-threonine protein kinase to transduce the unfolded protein signal from the ER to the nucleus. IRE1p also has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
REFERENCES:1) Little et al. Crit. Rev. Eukaryot. Gene Expr. 1994;4:1-18.
2) Lee. Methods 2005;35:373-81.
3) Bertolotti et al. Nat. Cell Biol. 2000;2:326-32.
4) Shen et al. Dev. Cell 2002;3:99-111.
psi-iconCITATIONS
CITATIONS: 1)Wang et al. 20(S)-protopanaxadiol induces apoptosis in human umbilical vein endothelial cells by activating the PERK-eIF2alpha-ATF4 signaling pathway. J Cell Biochem. 2019;120(4):5085-5096.PMID: 30259568
2)Zhang et al. 20(S)-protopanaxadiol activates ER stress to induce cell apoptosis in human umbilical vein endothelial cells. Journal of Shanghai University of Traditional Chinese Medicine 2015;29(3):70-75.PMID:

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