Datasheet

IRAK2 Antibody
CATALOG NUMBER: 3595

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rat: (88%), Bovine: (82%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:IRAK2 antibody can be used for detection of IRAK2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1283 - RAW 264.7 Cell Lysate
 2) Cat. No. 1288 - A20 Cell Lysate
 3) Cat. No. 17-208 - A-20 Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 68 kDa

Observed: 65 kDa
SPECIFICITY:At least four isoforms of IRAK2 are known to exist; this antibody will detect all four isoforms. Anti-IRAK2 has no cross response to IRAK.
IMMUNOGEN:IRAK2 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of mouse IRAK2a.

The immunogen is located within the last 50 amino acids of IRAK2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IRAK2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IRAK2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IRAK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IRAK2 Antibody: IRAK-2, AI649099, 6330415L08Rik, Interleukin-1 receptor-associated kinase-like 2, IRAK-2
ACCESSION NO.:AAO24761
PROTEIN GI NO.:37725373
OFFICIAL SYMBOL:Irak2
GENE ID:108960

Background

BACKGROUND:IRAK2 Antibody: The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-κB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family has been identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-κB activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. Unlike human IRAK2, murine IRAK2 exists as four alternately spliced isoforms (IRAK2a-d), with two isoforms (IRAK2c and d) acting in an inhibitory fashion. IRAK2 is expressed in a variety of tissues.
REFERENCES: 1) Muzio M, Ni J, Feng P, et al. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997; 278:1612-5.
2) Zhang FX, Kirschning CJ, Mancinelli R, et al. Bacterial lipopolysaccharide activates nuclear factor-κB through interleukin-1 signaling mediators in cultured human dermal endothelial cells and mononuclear phagocytes. J. Biol. Chem. 1999; 274:7611-4.
3) Yang RB, Mark MR, Gurney AL, et al. Signaling events induced by lipopolysaccharide-activated toll-like receptor 2. J. Immunol. 1999; 163:639-43.
4) Hardy MP and O’Neill LAJ. The murine IRAK2 gene encodes four alternately spliced isoforms, two of which are inhibitory. J. Biol. Chem. 2004; 279:27699-708.

For Research Use Only