Datasheet

IRAK-M Antibody
CATALOG NUMBER: 2355

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:IRAK-M antibody can be used for detection of IRAK-M by Western blot at 1 μg/mL. IRAK-M antibody can also detect IRAK-M by immunohistochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in mouse and rat samples; Immunohistochemistry in human and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1406 - Mouse Spleen Tissue Lysate
 2) Cat. No. 1465 - Rat Kidney Tissue Lysate
 3) Cat. No. 10-901 - Human Spleen Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 66 kDa

Observed: 70 kDa
SPECIFICITY:IRAK-M antibody is predicted to have no cross reactivity to other members of the IRAK family.
IMMUNOGEN:IRAK-M antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IRAK-M.

The immunogen is located within the last 50 amino acids of IRAK-M.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IRAK-M Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IRAK-M Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IRAK-M antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IRAK-M Antibody: ASRT5, IRAKM, Interleukin-1 receptor-associated kinase 3, IL-1 receptor-associated kinase M, IRAK-3
ACCESSION NO.:NP_009130
PROTEIN GI NO.:5225377
OFFICIAL SYMBOL:IRAK3
GENE ID:11213

Background

BACKGROUND:IRAK-M Antibody: Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor-associated kinase (IRAK and IRAK2) mediates the activation of NF-betaB by IL-1/Toll receptors, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-M. IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and the dominant negative mutants of IRAKs inhibit IL-1 or LPS induced NF-betaB activation. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses to cytokine IL-1 and LPS.
REFERENCES: 1) Cao Z, Henzel WJ, and Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996; 271:1128-31.
2) Muzio M, Ni J, Feng P, et al. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997; 278:1612-5.
3) Wesche H, Gao X, Li X, et al. IRAK-M is a novel member of the Pelle/interleukin-1 receptor-associated kinase (IRAK) family. J. Biol. Chem. 1999; 274:19403-10.

For Research Use Only