| HOST SPECIES: | Mouse |
| SPECIES REACTIVITY: | Human, Mouse |
| IMMUNOGEN: | Recombinant protein corresponding to amino acids 618 - 712 of human IRAK. |
| TESTED APPLICATIONS: | ELISA, IHC-P, WB |
| APPLICATIONS: | IRAK antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested. |
| POSITIVE CONTROL: | 1) Cat. No. 95-112 - IRAK Recombinant Protein |
Specifications| PURIFICATION: | IRAK Monoclonal Antibody is immunoaffinity chromotography purified IgG. |
| CLONALITY: | Monoclonal |
| ISOTYPE: | IgG1 |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | IRAK Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | IRAK monoclonal antibody can be stored at -20˚C, stable for one year. |
| OFFICIAL SYMBOL: | IRAK1 |
| ALTERNATE NAMES: | IRAK Antibody [8F1A7] : IRAK, pelle |
| ACCESSION NO.: | P51617 |
| PROTEIN GI NO.: | 8928535 |
| GENE ID: | 3654 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | IRAK Monoclonal Antibody: Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-κB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF). |
| REFERENCES: | 1) Cao Z,; Henzel WJ, and Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996; 271:1128-31. | 2) Trofimova M, Sprenkle AB, Green M; et al. Developmental and tissue-specific expression of mouse pelle-like protein kinase. J. Bio. Chem. 1996; 271: 17609-12. | 3) Jianing Huang, Xiong Gao, Shyun Li, et al. Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin-1 receptor accessory protein. Proc. Natl. Acad. Sci. USA 1997; 94:12829-32. | 4) Robinson D, Shibuya K, Mui A, et al. IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NF-kB. Immunity 1997; 7:571-81. |
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