Datasheet

IRAK-4 Antibody
CATALOG NUMBER: 3125

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (85%), Mouse: (91%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:IRAK-4 antibody can be used for the detection of IRAK-4 by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1204 - K562 Cell Lysate
 2) Cat. No. 17-004 - K-562 Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 51 kDa

Observed: 51 kDa
SPECIFICITY:IRAK-4 antibody is predicted to not cross-react with other members of the IRAK protein family.
IMMUNOGEN:IRAK-4 antibody was raised against a synthetic peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK-4.

The immunogen is located within the last 50 amino acids of IRAK-4.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IRAK-4 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IRAK-4 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IRAK-4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IRAK-4 Antibody: IPD1, REN64, IRAK-4, NY-REN-64, Interleukin-1 receptor-associated kinase 4, Renal carcinoma antigen NY-REN-64
ACCESSION NO.:AAM15772
PROTEIN GI NO.:20219010
OFFICIAL SYMBOL:IRAK4
GENE ID:51135

Background

BACKGROUND:IRAK-4 Antibody: Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular responses through IL-1 receptor (IL-1R) and Toll-like receptors (TLR). IL-1R-associated kinases (IRAK, IRAK2, and IRAK-M) regulate the activation of NF-κB and MAP kinase (MAPK) by IL-1R/TLR. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-4. Overexpression of IRAK-4 activates NF-κB and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.
REFERENCES: 1) Cao Z, Henzel WJ, and Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996; 271:1128-31.
2) Muzio M, Ni J, Feng P, et al. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997; 278:1612-5
3) Wesche H, Gao X, Li X, et al. IRAK-M is a novel member of the Pelle/interleukin-1 receptor-associated kinase (IRAK) family. J. Biol. Chem. 1999; 274:19403-10.
4) Li S, Strelow A, Fontana EJ, et al. IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase. Proc. Natl. Acad. Sci. USA 2002; 99:5567-72.

For Research Use Only