IQSEC2 Antibody Cat. No.: 8011

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: IQSEC2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human IQSEC2.

The immunogen is located within amino acids 1380 - 1430 of IQSEC2.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: IQSEC2 antibody can be used for detection of IQSEC2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
SPECIFICITY: IQSEC2 antibody is human, mouse and rat reactive. At least two isoforms of IQSEC2 are known to exist; this antibody will only detect the larger isoform. IQSEC2 antibody is predicted to not cross-react with IQSEC1.
POSITIVE CONTROL:1) Cat. No. 1220 - SK-N-SH Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 104, 133, 141, 164 kDa

Observed: 140 kDa

psi-iconProperties
PURIFICATION:IQSEC2 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:IQSEC2 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IQSEC2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:IQSEC2
ALTERNATE NAMES:IQ motif and Sec7 domain 2, Brefeldin-resistant Arf-GEF 1 protein, BRAG1, MRX1
ACCESSION NO.:NP_001104595
PROTEIN GI NO.:162138911
GENE ID:23096
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:ADP ribosylation factors (Arfs) are small GTP-binding proteins known for their role in vesicular transport, where they nucleate the assembly of coat protein complexes at sites of carrier vesicle formation. Similar to the related protein IQSEC1, IQSEC2 is a guanine nucleotide exchange protein for ARF1 and ARF6 that belongs to the BRAG family and contains one IQ domain, one PH domain and one SEC7 domain (1,2). IQSEC2 is a major component of the postsynaptic density (PSD) and colocalizes with PSD-95. It has been suggested to modulate the functions of the ARF proteins (1). Mutations in this gene have been implicated in nonsyndromic X-linked mental retardation (3).
REFERENCES:1) Murphy JA, Jensen ON, and Walikonis RS. BRAG1, a SEC7 domain-containing protein, is a component of the postsynaptic density of excitatory synapses. Brain Res. 2006; 1120:35-45.
2) Someya A, Sata M, Takeda K, et al. ARF-GEP(100), a guanine nucleotide-exchange protein for ADP-ribosy
3) Shoubridge C, Tarpey PS, Abidi F, et al. Mutations in the guanine nucleotide exchange factor gene IQSEC2 cause nonsyndromic intellectual ability. Nat. Genet. 2010; 42:486-8.

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