Datasheet

IQSEC1 Antibody
CATALOG NUMBER: 8009

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:IQSEC1 antibody can be used for detection of IQSEC1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry at 2 μg/mL. For immunofluorescence start at 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1288 - A20 Cell Lysate
 2) Cat. No. 17-208 - A-20 Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 106 kDa

Observed: 103 kDa
SPECIFICITY:IQSEC1 antibody is human, mouse and rat reactive. At least four isoforms of IQSEC1 are known to exist. IQSEC1 antibody is predicted to not cross-react with IQSEC2.
IMMUNOGEN:IQSEC1 antibody was raised against a 19 amino acid peptide near the amino terminus of human IQSEC1.

The immunogen is located within amino acids 60 - 110 of IQSEC1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IQSEC1 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IQSEC1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IQSEC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IQ motif and Sec7 domain 1, ADP-ribosylation factors guanine nucleotide-exchange protein 100, ARFGEP100, BRAG2, Brefeldin-resistant Arf-GEF 2 protein
ACCESSION NO.:NP_055684
PROTEIN GI NO.:50582989
OFFICIAL SYMBOL:IQSEC1
GENE ID:9922

Background

BACKGROUND:ADP ribosylation factors (Arfs) are small GTP-binding proteins known for their role in vesicular transport, where they nucleate the assembly of coat protein complexes at sites of carrier vesicle formation. IQSEC1 (GEP100/BRAG2), a guanine nucleotide exchange protein for ARF6, belongs to the BRAG family and contains one IQ domain, one PH domain and one SEC7 domain (1). As a known activator of ARF6, IQSEC1 regulates cell surface levels of Beta1 integrin and its knockdown blocked tumour metastasis (2,3). IQSEC1 links EGFR signaling to ARF6 activation and may contribute to the cancer metastasis and malignancy (4).
REFERENCES: 1) Someya A, Sata M, Takeda K, et al. ARF-GEP(100), a guanine nucleotide-exchange protein for ADP-ribosylation factor 6. Proc. Natl. Acad. Sci. U.S.A. 2001; 98:2413-8.
2) Dunphy JL, Moravec R, Ly K, et al. The Arf6 GEF GEP100/BRAG2 regulates cell adhesion by controlling endocytosis of beta1 integrins. Curr. Biol. 2006; 16:315-20.
3) Moravec R, Conger KK, D'Souza R, et al. BRAG2/GEP100/IQSec1 interacts with clathrin and regulates a5B1 integrin endocytosis through activation of ADP ribosylation factor 5 (Arf5). J. Biol. Chem. 2012; 287:31138-47.
4) Hu Z, Du J, Yang L, et al. GEP100/Arf6 is required for epidermal growth factor-induced ERK/Rac1 signaling and cell migration in human hepatoma HepG2 cells. PLoS One 2012; 7:e38777.

For Research Use Only