Datasheet

IPR1 Antibody
CATALOG NUMBER: 4407

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:Ipr1 antibody can be used for detection of Ipr1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
IMMUNOGEN:IPR1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human IPR1.

The immunogen is located within amino acids 470 - 520 of IPR1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IPR1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IPR1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IPR1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IPR1 Antibody: IPR1, VODI, IFI41, IFI75, Sp110 nuclear body protein, Speckled 110 kDa
ACCESSION NO.:NP_004501
PROTEIN GI NO.:190343008
OFFICIAL SYMBOL:SP110
GENE ID:3431

Background

BACKGROUND:IPR1 Antibody: Susceptibility to tuberculosis (TB) in mice has recently been attributed to the IPR1 gene. IPR1 is a member of the SP100/SP140 family of nuclear body proteins and encodes a leukocyte-specific nuclear body component. The protein can function as an activator of gene transcription and may serve as a nuclear hormone receptor coactivator. Alternative splicing has been observed for this gene and three transcript variants, encoding distinct isoforms, have been identified. SP110 is the closest homolog of the IPR1 protein in humans. The IPR1/Sp110 gene product might play a role in integrating signals generated by intracellular pathogens with mechanisms controlling innate immunity, cell death, and pathogenesis. IPR1/Sp110 is up-regulated after infection with M. tuberculosis and required by Anaplasma phagocytophilum for infection of human promyelocytic cells. Defects in Sp110 are a cause of severely impaired resistance to infection by M. tuberculosis.
REFERENCES: 1) Pan H, Yan BS, Rojas M, et al. Ipr1 gene mediates innate immunity to tuberculosis. Nature2005; 434:767-72.
2) Bloch DB, Nakajima A, Gulick T, et al. Sp110 localizes to the PML-Sp100 nuclear body and may function as a nuclear hormone receptor transcriptional coactivator. Mol. Cell Biol.2000; 20:6138-46.
3) De la Fuente J, Manzano-Roman R, Blouin EF, et al. Sp110 transcription is induced and required by Anaplasma phagocytophilum for infection of human promyelocytic cells. BMC Infect. Dis.2007; 7:110.
4) Tosh K. Campbell SJ, Fielding K, et al. Variants in the SP110 gene are associated with genetic susceptibility to tuberculosis in West Africa. Proc. Natl. Acad. Sci.2006; 103:10364-8.

For Research Use Only