Ipaf Antibody Cat. No.: 3107

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (79%), Mouse: (71%)
IMMUNOGEN: Ipaf antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of human Ipaf.

The immunogen is located within the last 50 amino acids of Ipaf.
TESTED APPLICATIONS: ELISA, ICC, IF, WB
APPLICATIONS: Ipaf polyclonal antibody can be used for the detection of Ipaf by Western blot at 1 and 2 μg/mL. A 110 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1371 - Human peripheral blood leukocyte (PBL) Lysate
 2) Cat. No. 1208 - THP-1 Cell Lysate
 3) Cat. No. 17-008 - THP-1 Cell Slide
PREDICTED MOLECULAR WEIGHT: 110 kDa

psi-iconProperties
PURIFICATION:Ipaf Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Ipaf Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Ipaf antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:NLRC4
ALTERNATE NAMES:Ipaf Antibody: CLAN, IPAF, CLAN1, CLANA, CLANB, CLANC, CLAND, CARD12, CLR2.1, CLAN, UNQ6189/PRO20215, NLR family CARD domain-containing protein 4, CARD, LRR, and NACHT-containing protein, Clan protein
ACCESSION NO.:NP_067032
PROTEIN GI NO.:40788015
GENE ID:58484
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Ipaf Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adaptor molecules and proteases including several members of the caspase family. Another family of proteins that functions as a critical regulator of apoptosis and NFκ signaling pathways is the CED-4/Apaf-1 (apoptosis protein activating factor-1) protein family. Ipaf (ICE protease activating factor) is a CED-4/Apaf-1 family member that activates caspase-1/ICE and can induce apoptosis in human cells in a caspase-1 dependent manner. Ipaf and caspase-1 are thought to interact with each other through the association of the Ipaf amino-terminal CARD (caspase recruitment domain) and amino-terminal CARD of caspase-1.
REFERENCES:1) Li P, Nijhawan D, Budihardjo I, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997; 91:479-89.
2) Poyet J-L, Srinivaula SM, Tnani M, et al. Identification of Ipaf, a human caspase-1-activating protein related to Apaf-1. J. Biol. Chem. 2001; 276:28309-13.
3) Geddes BJ, Wang L, Huang WJ, et al. Human CARD12 is a novel CED4/Apaf-1 family member that induces apoptosis. Biochem. Biophys. Res. Commun. 2001; 284:77-82.

ANTIBODIES FOR RESEARCH USE ONLY.

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