Integrin alpha 4 Antibody Cat. No.: 4837

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Integrin alpha 4 antibody was raised against a 15 amino acid synthetic peptide from near the center of human Integrin alpha 4.

The immunogen is located within amino acids 220 - 270 of Integrin alpha 4.
TESTED APPLICATIONS: ELISA, IF, WB
APPLICATIONS: Integrin alpha 4 antibody can be used for the detection of Integrin alpha 4 by Western blot at 1 μg/mL. Despite its predicted molecular weight, Integrin alpha4 will often run at higher molecular weight in SDS-PAGE. Antibody can also be used for immunoflourescence starting at 2 μg/mL.

Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: At least two isoforms are known to exist. The immunogen to this antibody is within the Integrin alpha4 recombinant protein fragment and thus will recognize it.
POSITIVE CONTROL:1) Cat. No. 1466 - Rat Spleen Tissue Lysate
 2) Cat. No. 17-005 - Jurkat Cell Slide

psi-iconProperties
PURIFICATION:Integrin alpha 4 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Integrin alpha 4 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Integrin alpha 4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:ITGA4
ALTERNATE NAMES:Integrin alpha 4 Antibody: IA4, CD49D, Integrin alpha-4, CD49 antigen-like family member D
ACCESSION NO.:NP_000876
PROTEIN GI NO.:67191027
GENE ID:3676
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Integrin alpha 4 Antibody: The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.
REFERENCES:1) Wegener KL and Campbell ID. Transmembrane and cytoplasmic domains in integrin activation and protein-protein interactions. Mol. Membr. Biol.2008; 25:376-87.
2) Kamata T, Puzon W, and Takada Y. Identification of putative ligand-binding sites of the integrin alpha 4 beta 1 (VLA-4, CD49d/CD29). Biochem J.1995; 305:945-51.
3) Berlin C, Berg EL, Briskin MJ, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell1993; 74:185-95.
4) Pulido R, Elices MJ, Campanero MR, et al. Functional evidence for three distinct and independently inhibitable adhesion activities mediated by the human integrin VLA-4. Correlation with distinct alpha 4 epitopes. J. Biol. Chem.1991; 266:10241-5.

ANTIBODIES FOR RESEARCH USE ONLY.

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