| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| IMMUNOGEN: | INPP4B antibody was raised against a 17 amino acid peptide near the amino terminus of human INPP4B. The immunogen is located within the last 50 amino acids of INPP4B. |
| TESTED APPLICATIONS: | ELISA, ICC, WB |
| APPLICATIONS: | INPP4B antibody can be used for detection of INPP4B by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested. |
| SPECIFICITY: | INPP4B antibody is human, mouse and rat reactive. At least two isoforms of INPP4B are known to exist; this antibody only detects the longer isoform. INPP4B antibody is predicted to not cross-react with INPP4A. |
| POSITIVE CONTROL: | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
| 2) Cat. No. 17-201 - 3T3/BALB Cell Slide | |
| PREDICTED MOLECULAR WEIGHT: | Predicted: 102 kDa Observed: 100 kDa |
Specifications| PURIFICATION: | INPP4B antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | INPP4B antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | INPP4B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| OFFICIAL SYMBOL: | INPP4B |
| ALTERNATE NAMES: | Inositol polyphosphate-4-phosphatase type II 105kDa |
| ACCESSION NO.: | NP_003857 |
| PROTEIN GI NO.: | 156104895 |
| GENE ID: | 8821 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | Inositol polyphosphate 4-phosphatase type II (INPP4B) is one of the enzymes involved in phosphatidylinositol signaling pathways. It removes the phosphate group at position 4 of the inositol ring from inositol 3,4-bisphosphate (1). INPP4B can function as a tumor suppressor by inhibiting PI3K signaling. In human epithelial cells, knockdown of INPP4B expression resulted in enhanced Akt activation, anchorage-independent growth, and enhanced overall motility (2). It is thought INPP4B may also have oncogenic functions as INPP4B-dependent PI3K signaling is mediated by SGK3 in breast cancer cells and expression of INPP4B leads to enhanced SGK3 activation and suppression of AKT phosphorylation (3). |
| REFERENCES: | 1) Norris FA, Auethavekiat V, and Majerus PW. The isolation and characterization of cDNA encoding human and rat brain inositol polyphosphate 4-phosphase. J. Biol. Chem. 1995; 270:16128-33. | 2) Gewinner C, Wang ZC, Richardson A, et al. Evidence that inositol polyphosphate 4-phosphase type II is a tumor suppressor that inhibits PI3K signaling. Cancer Cell 2009; 16:115-25. | 3) Gasser JA, Inuzuka H, Lau AW, et al. SGK3 mediates INPP4B-dependent PI3K signaling in breast cancer. Mol. Cell 2014; 56:595-607. |
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