INPP4B Antibody Cat. No.: 8339

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: INPP4B antibody was raised against a 17 amino acid peptide near the amino terminus of human INPP4B.

The immunogen is located within the last 50 amino acids of INPP4B.
TESTED APPLICATIONS: ELISA, ICC, WB
APPLICATIONS: INPP4B antibody can be used for detection of INPP4B by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
SPECIFICITY: INPP4B antibody is human, mouse and rat reactive. At least two isoforms of INPP4B are known to exist; this antibody only detects the longer isoform. INPP4B antibody is predicted to not cross-react with INPP4A.
POSITIVE CONTROL:1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
 2) Cat. No. 17-201 - 3T3/BALB Cell Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 102 kDa

Observed: 100 kDa

psi-iconProperties
PURIFICATION:INPP4B antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:INPP4B antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:INPP4B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:INPP4B
ALTERNATE NAMES:Inositol polyphosphate-4-phosphatase type II 105kDa
ACCESSION NO.:NP_003857
PROTEIN GI NO.:156104895
GENE ID:8821
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Inositol polyphosphate 4-phosphatase type II (INPP4B) is one of the enzymes involved in phosphatidylinositol signaling pathways. It removes the phosphate group at position 4 of the inositol ring from inositol 3,4-bisphosphate (1). INPP4B can function as a tumor suppressor by inhibiting PI3K signaling. In human epithelial cells, knockdown of INPP4B expression resulted in enhanced Akt activation, anchorage-independent growth, and enhanced overall motility (2). It is thought INPP4B may also have oncogenic functions as INPP4B-dependent PI3K signaling is mediated by SGK3 in breast cancer cells and expression of INPP4B leads to enhanced SGK3 activation and suppression of AKT phosphorylation (3).
REFERENCES:1) Norris FA, Auethavekiat V, and Majerus PW. The isolation and characterization of cDNA encoding human and rat brain inositol polyphosphate 4-phosphase. J. Biol. Chem. 1995; 270:16128-33.
2) Gewinner C, Wang ZC, Richardson A, et al. Evidence that inositol polyphosphate 4-phosphase type II is a tumor suppressor that inhibits PI3K signaling. Cancer Cell 2009; 16:115-25.
3) Gasser JA, Inuzuka H, Lau AW, et al. SGK3 mediates INPP4B-dependent PI3K signaling in breast cancer. Mol. Cell 2014; 56:595-607.

ANTIBODIES FOR RESEARCH USE ONLY.

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