TIM-3 Immune Checkpoint
TIM-3 Antibody: The immune checkpoint protein TIM-3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-γ producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1).TIM-3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2,3).
Match Pair Sets
A sandwich ELISA was performed using the anti-TIM-3 mAb RF16104 as the capture antibody with Biotin-labeled Risk-Free™ anti-TIM-3 mAb RF16103-biotin as the detection antibody.
| Target | Cat. No. | Capture | Detection | Host | Datasheet |
|---|---|---|---|---|---|
| TIM-3 Antibody Pair *See Capture ELISA Data*  |
RFP-1001 |
RF16104 | RF16103-Biotin | Mouse - Mouse |
PDF
Risk-Free™ Monoclonal TIM-3 Products
ProSci's Risk-Free™ antibodies are monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Risk-Free™ means they are guaranteed to work for their approved applications.
"Hover over research application to see respective image (if available)."
Risk-Free™ Mouse Monoclonal TIM-3 Set
ProSci's Risk-Free™ Sets consists of several mAbs with different binding features allowing selection of the best clone.
| Product Set | Size | Contents | Description |
|---|---|---|---|
| Risk-Free TIM-3 Set (RF16100) | 20μg / Clone | RF16103, RF16104, RF16105, RF16106, RF16107, RF16108, RF16109 | TIM-3 antibodies were raised against the extracellular domain of human TIM-3. |
Risk-Free™ Mouse Monoclonal TIM-3 Antibodies
ProSci Risk-Free™ TIM-3 products are rigorously tested and guaranteed to work for their designated applications.
| Cat. No. | ELISA | WB | ICC | IF | IHC | Flow | Conjugation |
|---|---|---|---|---|---|---|---|
| RF16103 Host Species: Mouse Reactivity: Human |
 ELISA Titration Curve Analysis |
 WB in HEK293 Cells |
 ICC in HEK293 Cells |
 IF in HEK293 Cells |
 IHC in Human Stomach Carcinoma |
 Flow in HEK293 Cells |
Available |
| RF16104 Host Species: Mouse Reactivity: Human |
ELISA Titration Curve Analysis |
 ICC in HEK293 Cells |
 Flow in HEK293 Cells |
Available | |||
| RF16105 Host Species: Mouse Reactivity: Human |
ELISA Titration Curve Analysis |
 ICC in HEK293 Cells |
 IF in HEK293 Cells |
 IHC in Human Stomach Carcinoma |
 Flow in HEK293 Cells |
Available | |
| RF16106 Host Species: Mouse Reactivity: Human |
ELISA Titration Curve Analysis |
WB in HEK293 Cells |
 ICC in HEK293 Cells |
 IF in HEK293 Cells |
 IHC in Human Stomach Carcinoma |
 Flow in HEK293 Cells |
Available |
| RF16107 Host Species: Mouse Reactivity: Human |
ELISA Titration Curve Analysis |
 WB in HEK293 Cells |
 ICC in HEK293 Cells |
 IF in HEK293 Cells |
 IHC in Human Stomach Carcinoma |
 Flow in HEK293 Cells |
Available |
| RF16108 Host Species: Mouse Reactivity: Human |
ELISA Titration Curve Analysis |
 ICC in HEK293 Cells |
IF in HEK293 Cells |
 IHC in Human Stomach Carcinoma |
 Flow in HEK293 Cells |
Available | |
| RF16109 Host Species: Mouse Reactivity: Human |
ELISA Titration Curve Analysis |
ICC in HEK293 Cells |
 IF in HEK293 Cells |
 IHC in Human Stomach Carcinoma |
 Flow in HEK293 Cells |
Available |
= Best Application
Risk-Free™ Single Domain Monoclonal TIM-3 Products
Llama single domain antibodies (sdAbs) offer unique characteristics amenable to many applications. These sdAbs demonstrate low nanomolar binding affinity and can be used for the various applications. Note: TIM-3 single domain antibody should be stored in working aliquots at -20 °C, stable for up to one year. As with all antibodies, care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Coming Soon
If you want to be notified when this target will be released, let us know.
Related Products
Antigens
| Cat. No. | Product Type | Species | Tissue/Cell | Datasheet |
|---|---|---|---|---|
| RF16100-01 | Recombinant Protein | Human | HEK293 |
Lysates
| Cat. No. | Product Type | Species | Tissue/Cell | Datasheet |
|---|---|---|---|---|
| N/A | N/A |
Slides
Conjugates
| Cat. No. | Biotin | Custom | |
|---|---|---|---|
| RF16103-Biotin | Available | Contact Us | |
| RF16104-Biotin | Inquire | Contact Us | |
| RF16105-Biotin | Inquire | Contact Us | |
| RF16106-Biotin | Inquire | Contact Us | |
| RF16107-Biotin | Inquire | Contact Us | |
| RF16108-Biotin | Inquire | Contact Us | |
| RF16109-Biotin | Inquire | Contact Us | |
Additional TIM-3 Antibody Products
Monoclonal antibodies are highly specific and recognize only one epitope on an antigen which generates consistent performance in quantitative detection applications regardless of production batch. Polyclonal antibodies have high affinity and recognize different epitopes on a specific antigen. Note: These antibodies can be stored at -20˚C and are stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
TIM-3 Monoclonal Products
| Cat. No. | Name | Applications | Datasheet |
|---|---|---|---|
| 77-026Host Species: Mouse Reactivity: Human |
TIM-3 Antibody [F38-2E2](APC) | Flow | |
| 77-027Host Species: Mouse Reactivity: Mouse |
TIM3 Antibody [F38-2E2] (APC) | Flow | |
| 36-028Host Species: Rat Reactivity: Mouse |
Tim-3 Antibody [T1 339H] | E, WB | |
| 36-029Host Species: Rat Reactivity: Mouse |
Tim-3 Antibody [TI 142F] | E, Flow |
TIM-3 Polyclonal Products
| Cat. No. | Antigen Range | ELISA | WB | ICC | IF | IHC | Flow | Datasheet |
|---|---|---|---|---|---|---|---|---|
| 8659 Host Species: Rabbit Reactivity: HU |
12 - 142 |  WB in Jurkat cell lysate at 1 μg/mL |
 |
 |
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| 46-495 | ||||||||
| 60-816 |
TIM-3 Recombinant Protein Products
| Cat. No. | Sequence | Fusion Tag | Species | Tissue/Cell | Datasheet |
|---|---|---|---|---|---|
| 96-723 | Ser 22 - Arg 200 | His Tag | Human | HEK293 | |
| 96-724 |
Ser 22 - Arg 200 | C-Fc Tag | Human | HEK293 | |
| 96-491 | Ser 22 - Glu 142 | C-Fc Tag | Human | HEK293 | |
| 91-327 | Ser 22 - Arg 200 | C-6 His tag | Human | Human Cells | |
| 91-942 | Ser 22 - Arg 200 | C-Fc-6 His tag | Human | Human Cells | |
| 92-656 | Glu 24 - Ile193 | C-6 His tag | Marmoset | Human Cells | |
| 92-552 | Leu 22 - Ala193 | C-6 His tag | Mouse | Human Cells | |
| 92-596 | Arg 20 - Arg191 | C-Fc tag | Mouse | Human Cells | |
| 92-646 | Arg 20 - Arg191 | C-6 His tag | Mouse | Human Cells | |
| 90-549 | aa 22-200 | Fc Tag | Human | CHO cells | |
| 90-550 | aa 21-189 | Fc Tag | Human | CHO cells |
