TIM-3 Immune Checkpoint

 

TIM-3 Antibody: The immune checkpoint protein TIM-3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-γ producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1).TIM-3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2,3).


Match Pair Sets

A sandwich ELISA was performed using the anti-TIM-3 mAb RF16104 as the capture antibody with Biotin-labeled Risk-Free™ anti-TIM-3 mAb RF16103-biotin as the detection antibody.

TargetCat. No.CaptureDetectionHostDatasheet
TIM-3 Antibody Pair
*See Capture ELISA Data*TIM-3 Matched Pair ELISA Data
RFP-1001
RF16104 RF16103-Biotin Mouse - Mouse

Risk-Free™ Monoclonal TIM-3 Products

ProSci's Risk-Free™ antibodies are monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Risk-Free™ means they are guaranteed to work for their approved applications.
"Hover over research application to see respective image (if available)."

Risk-Free™ Mouse Monoclonal TIM-3 Set

ProSci's Risk-Free™ Sets consists of several mAbs with different binding features allowing selection of the best clone.

Product SetSizeContentsDescription
Risk-Free TIM-3 Set (RF16100) 20μg / Clone RF16103, RF16104, RF16105, RF16106, RF16107, RF16108, RF16109 TIM-3 antibodies were raised against the extracellular domain of human TIM-3.

Risk-Free™ Mouse Monoclonal TIM-3 Antibodies

ProSci Risk-Free™ TIM-3 products are rigorously tested and guaranteed to work for their designated applications.

= Negative = Fair   = Average    = Good     = Excellent= Best Application
Cat. No.ELISAWBICCIFIHCFlowConjugation
RF16103 Host Species: Mouse
Reactivity: Human
    
ELISA Titration Curve Analysis
  
WB in HEK293 Cells
  
ICC in HEK293 Cells

IF in HEK293 Cells

IHC in Human Stomach Carcinoma
   
Flow in HEK293 Cells
Available
RF16104 Host Species: Mouse
Reactivity: Human
    
ELISA Titration Curve Analysis
     
ICC in HEK293 Cells
         Flow cytometry analysis of TIM-3 over expressing HEK293 cells using (A) RF16082, (B) RF16083, (C) RF16084, (D) RF16086, (E) RF16087, (F) RF16088, (G) RF16089, and (H) control mouse IgG antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: TIM-3 over expressing HEK293 cells
Flow in HEK293 Cells
Available
RF16105 Host Species: Mouse
Reactivity: Human
   
ELISA Titration Curve Analysis
     
ICC in HEK293 Cells
  
IF in HEK293 Cells
  
IHC in Human Stomach Carcinoma
   
Flow in HEK293 Cells
Available
RF16106 Host Species: Mouse
Reactivity: Human
  
ELISA Titration Curve Analysis
  
WB in HEK293 Cells
  
ICC in HEK293 Cells
  
IF in HEK293 Cells
   
IHC in Human Stomach Carcinoma
   
Flow in HEK293 Cells
Available
RF16107 Host Species: Mouse
Reactivity: Human
  
ELISA Titration Curve Analysis
  
WB in HEK293 Cells
  
ICC in HEK293 Cells
  
IF in HEK293 Cells
  
IHC in Human Stomach Carcinoma
   
Flow in HEK293 Cells
Available
RF16108 Host Species: Mouse
Reactivity: Human
  
ELISA Titration Curve Analysis
     
ICC in HEK293 Cells
   
IF in HEK293 Cells
  
IHC in Human Stomach Carcinoma
   
Flow in HEK293 Cells
Available
RF16109 Host Species: Mouse
Reactivity: Human
  
ELISA Titration Curve Analysis
     
ICC in HEK293 Cells
   
IF in HEK293 Cells
   
IHC in Human Stomach Carcinoma
   
Flow in HEK293 Cells
Available

Risk-Free™ Single Domain Monoclonal TIM-3 Products

Llama single domain antibodies (sdAbs) offer unique characteristics amenable to many applications. These sdAbs demonstrate low nanomolar binding affinity and can be used for the various applications. Note: TIM-3 single domain antibody should be stored in working aliquots at -20 °C, stable for up to one year. As with all antibodies, care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Coming Soon

  • Immunization
  • PBMC Isolation
  • Library Construction
  • Validation
  • Release

If you want to be notified when this target will be released, let us know.

Related Products

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Antigens


Cat. No.Product TypeSpeciesTissue/CellDatasheet
RF16100-01 Recombinant Protein Human HEK293
 
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Lysates


Cat. No.Product TypeSpeciesTissue/CellDatasheet
N/A N/A
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Slides


Cat. No.Product TypeSpeciesTissue/CellDatasheet
N/A
N/A
 
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Conjugates


Cat. No.BiotinCustom
RF16103-Biotin Available Contact Us
RF16104-Biotin Inquire Contact Us
RF16105-Biotin Inquire Contact Us
RF16106-Biotin Inquire Contact Us
RF16107-Biotin Inquire Contact Us
RF16108-Biotin Inquire Contact Us
RF16109-Biotin Inquire Contact Us
 

Additional TIM-3 Antibody Products

Monoclonal antibodies are highly specific and recognize only one epitope on an antigen which generates consistent performance in quantitative detection applications regardless of production batch. Polyclonal antibodies have high affinity and recognize different epitopes on a specific antigen. Note: These antibodies can be stored at -20˚C and are stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.


TIM-3 Monoclonal Products

Cat. No.NameApplicationsDatasheet
77-026Host Species: Mouse
Reactivity: Human
TIM-3 Antibody [F38-2E2](APC) Flow
77-027Host Species: Mouse
Reactivity: Mouse
TIM3 Antibody [F38-2E2] (APC) Flow
36-028Host Species: Rat 
Reactivity: Mouse
Tim-3 Antibody [T1 339H] E, WB
36-029Host Species: Rat 
Reactivity: Mouse
Tim-3 Antibody [TI 142F] E, Flow

TIM-3 Polyclonal Products

= Not Yet Determined = Negative = Fair   = Average    = Good     = Excellent
Cat. No.Antigen RangeELISAWBICCIFIHCFlowDatasheet
8659 Host Species: Rabbit
Reactivity: HU
12 - 142        
WB in Jurkat cell lysate at 1 μg/mL
       

    

  
46-495                       
60-816                       

TIM-3 Recombinant Protein Products

Cat. No.SequenceFusion TagSpeciesTissue/CellDatasheet
96-723 Ser 22 - Arg 200 His Tag Human HEK293
96-724
Ser 22 - Arg 200 C-Fc Tag Human HEK293
96-491 Ser 22 - Glu 142 C-Fc Tag Human HEK293
91-327 Ser 22 - Arg 200 C-6 His tag Human Human Cells
91-942 Ser 22 - Arg 200 C-Fc-6 His tag Human Human Cells
92-656 Glu 24 - Ile193 C-6 His tag Marmoset Human Cells
92-552 Leu 22 - Ala193 C-6 His tag Mouse Human Cells
92-596 Arg 20 - Arg191 C-Fc tag Mouse Human Cells
92-646 Arg 20 - Arg191 C-6 His tag Mouse Human Cells
90-549 aa 22-200 Fc Tag Human CHO cells
90-550 aa 21-189 Fc Tag Human CHO cells

Additional TIM-3 Information

 

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