Cat. No.: RF16070

VISTA Mouse mAb Detection Set (Risk-Free™)

VISTA Antibody: VISTA/B7-H5/platelet receptor Gi24 is a single-pass type I membrane protein located at the cell surface. It is an immunoregulatory receptor which can inhibit T-cell response and may promote differentiation of embryonic stem cells, by inhibiting the BMP4 signaling pathway. The protein can be cleaved by MMP14, and stimulate MMP14-mediated MMP2 activation.

Each Risk-Free™ monoclonal antibody set consists of several 20μg trial size mAbs with different binding features to help you find the best clone for your specific application. Go to set contents.

ProSci Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications.


Flow cytometry analysis of VISTA over expressing HEK293 cells using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: LIGHT overexpressing HEK293 cells

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Flow cytometry analysis of VISTA over expressing HEK293 cells using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: LIGHT overexpressing HEK293 cells.See high quality image.

Western blot analysis of VISTA in over expressing HEK293 cells using RF16071 antibody at(A) 0.25 μg/ml, (B) 0.5 μg/ml, and (C) 1 μg/ml

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Western blot analysis of VISTA in over expressing HEK293 cells using RF16071 antibody at (A) 0.25 μg/ml, (B) 0.5 μg/ml, and (C) 1 μg/ml. See high quality image.

Immunofluorescence of VISTA in over expressing HEK293 cells using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 2 μg/ml

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Immunofluorescence of VISTA in over expressing HEK293 cells using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 2 μg/ml. See high quality image.

Immunofluorescence of VISTA in human spleen tissue using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 10 μg/ml

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Immunofluorescence of VISTA in human spleen tissue using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 10 μg/ml. See high quality image.

Immunohistochemistry of VISTA in human lymphoma tissue using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 2 μg/ml

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Immunohistochemistry of VISTA in human lymphoma tissue using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 2 μg/ml. See high quality image.

Immunocytochemistry of VISTA in over expressing HEK293 cells using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 1 μg/ml

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Immunocytochemistry of VISTA in over expressing HEK293 cells using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 1 μg/ml. See high quality image.

Titration curve analysis of VISTA mAbs to detect recombinant LVISTA in ELISA with RF16071, RF16072, RF16073, and RF16074 abs at decreasing concentrations

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Titration curve analysis of VISTA mAbs to detect recombinant LVISTA in ELISA with RF16071,RF16072, RF16073, and RF16074 abs at decreasing concentrations. See high quality image.

VISTA Mouse Set contents

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VISTA Mouse Monoclonal Set Contents.

ProSci IconSpecifications

NameVISTA Detection Set (Risk-Free™)
Alternate Names VISTA Antibody: VSIR, B7-H5, B7H5, GI24, PP2135, SISP1, DD1alpha, VISTA, C10orf54, chromosome 10 open reading frame 54, PD-1H, V-set immunoregulatory receptor, V-Type Immunoglobulin Domain-Containing Suppressor Of T-Cell Activation, Chromosome 10 Open Reading Frame 54
Host Species Mouse
Tested Applications ELISA, Flow, ICC, IF, IHC-P, WB
Immunogen VISTA antibody was raised against the extracellular domain of human VISTA.
Size 20 μg per clone

ProSci IconProperties

Clonality Monoclonal
Isotype IgG1
Conjugate Unconjugated
Physical State Liquid
Buffer VISTA Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration 1 mg/mL
Storage Conditions Antibodies can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ProSci IconSet Contents

Hover over application title for rating comparison image.

= Negative = Fair   = Average    = Good     = Excellent= Best Application
Cat. No.ELISAELISA Titration Curve Analysis
ELISA Titration Curve Analysis
WBWestern Blot in HEK293 Cells
Western Blot in HEK293 Cells
ICCICC in HEK293 Cells
ICC in HEK293 Cells
IFIF in HEK293 Cells
IF in HEK293 Cells
IHCIHC in Human Lymphoma
IHC in Human Lymphoma
FlowFlow Cytometry in HEK293 Cells
Flow Cytometry in HEK293 Cells
RF16071                     
RF16072                      
RF16073                    
RF16074                     

ProSci IconProduct Specific Information

ProSci's Risk-Free™ antibodies are monoclonal antibodies made to improve in vitro studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. These antibodies have the following advantages:

  • Antibodies are developed against mammalian-expressed, post-translationally modified recombinant proteins.
  • Multiple antibodies per target allows the user to choose the best antibody for their application
  • Available individually or as a set
  • Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications:
    • • ELISA• Flow Cytometry• Immunoblot • Immunohistochemistry
      • Immunocytochemistry • Immunofluorescence

ProSci IconBackground

VISTA Antibody: VISTA/B7-H5/platelet receptor Gi24 is a single-pass type I membrane protein located at the cell surface. It is an immunoregulatory receptor which can inhibit T-cell response and may promote differentiation of embryonic stem cells, by inhibiting the BMP4 signaling pathway. The protein can be cleaved by MMP14, and stimulate MMP14-mediated MMP2 activation.


ProSci IconReferences

1) Mayya V., et al . Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. 2009, Sci. Signal. 2:RA46-RA46.
2) Sakr M.A., et al.,GI24 enhances tumor invasiveness by regulating cell surface membrane-type 1 matrix metalloproteinase. 2010, Cancer Sci. 101:2368-2374.


ANTIBODIES FOR RESEARCH USE ONLY.

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 Price Options

1 Set - $249.00

P4 Members save 10%!

Set consists of 20μg per clone.

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