Cat. No.: RF16120

TMIGD2 Mouse mAb Detection Set (Risk-Free™)

TMIGD2 Antibody: TMIGD2 (transmembrane and immunoglobulin domain containing 1), also known as immunoglobulin-containing and proline-rich receptor 1 (IGPR1), is novel adhesion molecule that is expressed in multiple tissues, primarily in cells of epithelium and endothelium origins (1). TMIGD2 is thought to be involved in angiogenesis and regulates cellular morphology, homophilic cell aggregation, and cell-cell interaction. TMIGD2 activity also modulates actin stress fiber formation and focal adhesion and reduces cell migration.

Each Risk-Free™ monoclonal antibody set consists of several 20μg trial size mAbs with different binding features to help you find the best clone for your specific application. Go to set contents.

ProSci Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications.


Western blot analysis of TMIGD2 in over expressing HEK293 cells using RF16121 and RF16122 antibodies at (A) 0.25 mg/ml , (B) 0.5 mg/ml,  and (C) 1 mg/ml

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Western blot analysis of TMIGD2 in over expressing HEK293 cells using RF16121 and RF16122 antibodies at (A) 0.25 mg/ml , (B) 0.5 mg/ml, and (C) 1 mg/ml. See high quality image.

Flow cytometry analysis of TMIGD2 in over expressing HEK293 cells using RF16121 and (B) mouse IgG at 10 mg/ml.  Blue:  untransfected HEK293 cells. Yellow: TMIGD2 over expressing HEK293 cells.

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Flow cytometry analysis of TMIGD2 in over expressing HEK293 cells using RF16121 and (B) mouse IgG at 10 mg/ml. Blue: untransfected HEK293 cells. Yellow: TMIGD2 over expressing HEK293 cells.See high quality image.

Immunofluorescence of TMIGD2 in human colon carcinoma tissue using (A) RF16121, (B) RF16122, (C) RF16123, and (D) control mouse IgG antibody at 20 μg/ml.

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Immunofluorescence of TMIGD2 in human colon carcinoma tissue using (A) RF16121, (B) RF16122, (C) RF16123, and (D) control mouse IgG antibody at 20 μg/ml. See high quality image.

Immunohistochemistry of TMIGD2 in human colon carcinoma tissue using RF16121, (B) RF16122, (C) RF16123, and (D) control mouse IgG antibody at 5 μg/ml

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Immunohistochemistry of TMIGD2 in human colon carcinoma tissue using RF16121, (B) RF16122, (C) RF16123, and (D) control mouse IgG antibody at 5 μg/ml. See high quality image.

Immunocytochemistry of TMIGD2 in transfected HEK293 cells using (A) RF16121, (B) RF16121,  (C) RF16123, and (D) control mouse IgG antibody at 2 μg/ml

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Immunocytochemistry of TMIGD2 in transfected HEK293 cells using (A) RF16121, (B) RF16121, (C) RF16123, and (D) control mouse IgG antibody at 2 μg/ml. See high quality image.

Titration curve analysis of TMIDG2 mAbs to detect recombinant TMIDG2 in ELISA with RF16011, RF16012, and RF16013 antibodies at decreasing concentrations

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Titration curve analysis of TMIDG2 mAbs to detect recombinant TMIDG2 in ELISA with RF16011, RF16012, and RF16013 antibodies at decreasing concentrations. See high quality image.

psi-iconSpecifications

NameTMIDG2 Detection Set (Risk-Free™)
Alternate Names TMIDG2 Antibody: CTL4, cytotoxic T-lymphocyte associated protein 4, CD, GSE, GRD4, ALPS5, CD152, IDDM12, CELIAC3
Host Species Mouse
Tested Applications ELISA, ICC, IF, IHC-P, WB
Immunogen Mouse monoclonal TMIDG2 antibodies were raised against the extracellular domain of human TMIDG2.
Size 20 μg per clone

ProSci IconProperties

Clonality Monoclonal
Isotype IgG1
Conjugate Unconjugated
Physical State Liquid
Buffer PBS containing 0.02% sodium azide.
Concentration 1 mg/mL
Storage Conditions Antibodies can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ProSci IconSet Contents

Hover over application title for rating comparison image.

= Negative = Fair   = Average    = Good     = Excellent= Best Application
Cat. No.ELISAELISA Titration Curve Analysis
ELISA Titration Curve Analysis
WB>Western Blot in HEK293 Cells
Western Blot in HEK293 Cells
ICCICC in HEK293 Cells
ICC in HEK293 Cells
IFIF in HEK293 Cells
IF in HEK293 Cells
IHCIHC in Human Lymph Node Tissue
IHC in Human Lymph Node Tissue
FlowIHC in Human Lymph Node Tissue
Flow in HEK293
RF16121                 
RF16122                    
RF16123                    

ProSci IconProduct Specific Information

ProSci's Risk-Free™ antibodies are monoclonal antibodies made to improve in vitro studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. These antibodies have the following advantages:

  • Antibodies are developed against mammalian-expressed, post-translationally modified recombinant proteins.
  • Multiple antibodies per target allows the user to choose the best antibody for their application
  • Available individually or as a set
  • Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications:
    • • ELISA• Flow Cytometry• Immunoblot • Immunohistochemistry
      • Immunocytochemistry • Immunofluorescence

ProSci IconBackground

TMIGD2 Antibody: TMIGD2 (transmembrane and immunoglobulin domain containing 1), also known as immunoglobulin-containing and proline-rich receptor 1 (IGPR1), is novel adhesion molecule that is expressed in multiple tissues, primarily in cells of epithelium and endothelium origins (1). TMIGD2 is thought to be involved in angiogenesis and regulates cellular morphology, homophilic cell aggregation, and cell-cell interaction. TMIGD2 activity also modulates actin stress fiber formation and focal adhesion and reduces cell migration.


ProSci IconReferences

1) Brunet JF, Denizot F, Luciani MF, et al. A new member of the immunoglobulin superfamily--TMIDG2 . Nature 1987; 328:267–70.
2) Walunas TL, Lenschow DJ, Bakker CY, et al. TMIDG2 can function as a negative regulator of T cell activation. Immunity 1994; 1:405–13.
3) Harding FA, McArthur JG, Gross JA, et al. CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones. Nature 1992; 356:607–9.
4) Romo-Tena J, Gomez-Martin D, and Alcocer-Verela J. TMIDG2 and autoimmunity: new insights into the dual regulator of tolerance. Autoimmun. Rev. 2013; 12:117.


ANTIBODIES FOR RESEARCH USE ONLY.

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1 Set - $199.00

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Set consists of 20μg per clone.

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