Cat. No.:RF16100

TIM-3 Mouse mAb Detection Set (Risk-Free™)

TIM-3 Antibody: The immune checkpoint protein TIM-3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-γ producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1). TIM-3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2,3).

Each Risk-Free™ monoclonal antibody set consists of several 20μg trial size mAbs with different binding features to help you find the best clone for your specific application. Go to set contents.

ProSci Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications.


Flow cytometry analysis of TIM-3 over expressing HEK293 cells using (A) RF16103, (B) RF16104, (C) RF16105, (D) RF16106, (E) RF16107, (F) RF16108, (G) RF16109, and (H) control mouse IgG antibody at 0.1 μg/ml. Blue: untransfected HEK293 cells. Yellow: TIM-3 over expressing HEK293 cells

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Flow cytometry analysis of TIM-3 over expressing HEK293 cells using (A) RF16103, (B) RF16104, (C) RF16105, (D) RF16106, (E) RF16107, (F) RF16108, (G) RF16109, and (H) control mouse IgG antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells. See high quality image.

Western blot analysis of TIM-3 in over expressing HEK293 cells using RF16103, RF16106, and RF16107 antibodies at (A) 0.25 μg/ml , (B) 0.5 μg/ml, and (C) 1 μg/ml

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Western blot analysis of TIM-3 in over expressing HEK293 cells using RF16103, RF16106, and RF16107 antibodies at (A) 0.25 μg/ml , (B) 0.5 μg/ml, and (C) 1 μg/ml. See high quality image.

Immunofluorescence of TIM-3 in over expressing HEK293 cells using (A) RF16103,(B) RF16105, (C) RF16106, (D) RF16107, (E) RF16108, (F) RF16109, (G) control mouse IgG antibody at 10 μg/ml.

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Immunofluorescence of TIM-3 in over expressing HEK293 cells using (A) RF16103,(B) RF16105, (C) RF16106, (D) RF16107, (E) RF16108, (F) RF16109, (G) control mouse IgG antibody at 10 μg/ml. See high quality image.

Immunofluorescence of TIM-3 in human colon carcinoma tissue using (A) RF16103, (B) RF16105, (C) RF16106, (D) RF16107, (E) RF16108, (F) RF16109, and (G) control mouse IgG antibody at 20 μg/ml

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Immunofluorescence of TIM-3 in human colon carcinoma tissue using (A) RF16103, (B) RF16105, (C) RF16106, (D) RF16107, (E) RF16108, (F) RF16109, and (G) control mouse IgG antibody at 20 μg/ml. See high quality image.

Immunocytochemistry of TIM-3 in over expressing HEK293 cells using (A) RF16103, (B) RF16104, (C) RF16105, (D) RF16106, (E) RF16107, (F) RF16108, (G) RF16109, and (H) control mouse IgG antibody at 1 μg/ml

(Click image to expand)
Immunocytochemistry of TIM-3 in over expressing HEK293 cells using (A) RF16103, (B) RF16104, (C) RF16105, (D) RF16106, (E) RF16107, (F) RF16108, (G) RF16109, and (H) control mouse IgG antibody at 1 μg/ml. See high quality image.

Immunohistochemistry of TIM-3 in human lymphoma tissue using (A) RF16103, (B) RF16105, (C) RF16106, (D) RF16107, (E) RF16108, (F) RF16109, and (G) control mouse IgG antibody at 5 μg/ml

(Click image to expand)
Immunohistochemistry of TIM-3 in human lymphoma tissue using (A) RF16103, (B) RF16105, (C) RF16106, (D) RF16107, (E) RF16108, (F) RF16109, and (G) control mouse IgG antibody at 5 μg/ml. See high quality image.

Titration curve analysis of TIM-3 mAbs to detect recombinant TIM-3 in ELISA with RF16103, RF16104, RF16105, RF16106, RF16107, RF16108, and RF16109 antibodies at decreasing concentrations.

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Titration curve analysis of TIM-3 mAbs to detect recombinant TIM-3 in ELISA with RF16103, RF16104, RF16105, RF16106, RF16107, RF16108, and RF16109 antibodies at decreasing concentrations. See high quality image.

TIM-3 Mouse Monoclonal Set Contents

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TIM-3 Mouse Monoclonal Set Contents.

ProSci IconSpecifications

NameTIM-3 Detection Set (Risk-Free™)
Alternate Names TIM-3 Antibody: Hepatitis A virus cellular receptor, HAVCR2, TIM3, CD366, KIM-3, TIMD3, TIMD-3
Host Species Mouse
Tested Applications ELISA, Flow, ICC, IF, IHC-P, WB
Immunogen Mouse monoclonal TIM-3 antibodies were raised against the extracellular domain of human TIM-3.
Size 20 μg per clone

Properties

Clonality Monoclonal
Isotype IgG1,IgG2b
Conjugate Unconjugated
Physical State Liquid
Buffer PBS containing 0.02% sodium azide.
Concentration 1 mg/mL
Storage Conditions Antibodies can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ProSci IconSet Contents

Hover over application title for rating comparison image.

<<
= Negative = Fair   = Average    = Good     = Excellent= Best Application
Cat. No.ELISA
ELISA Titration Curve Analysis
WB
Western Blot in HEK293 Cells
ICC
ICC in HEK293 Cells
IF
IF in HEK293 Cells
IHC
IHC in Human Colon Tissue
Flow
Flow Cytometry in HEK293 Cells
RF16103              
RF16104                   
RF16105                      
RF16106                          
RF16107                             
RF16108                    
RF16109                     

ProSci IconProduct Specific Information

ProSci's Risk-Free™ antibodies are monoclonal antibodies made to improve in vitro studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. These antibodies have the following advantages:

  • Antibodies are developed against mammalian-expressed, post-translationally modified recombinant proteins.
  • Multiple antibodies per target allows the user to choose the best antibody for their application
  • Available individually or as a set
  • Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications:
    • • ELISA• Flow Cytometry• Immunoblot • Immunohistochemistry
      • Immunocytochemistry • Immunofluorescence

ProSci IconBackground

TIM-3 Antibody: The immune checkpoint protein TIM-3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-γ producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1). TIM-3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2,3).


ProSci IconReferences

1) Monney L, Sabatos CA, Gaglia JL, et al. Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of autoimmune disease. Nature 2002; 415:536-41.
2) Sakuishi K, Apetoh L, Sullivan JM, et al. Targeting Tim-3 and PD-1 pathways to reverse T cell exhaustion and restore anti-tumor immunity. J Exp Med 2010; 207:2187–94.
3) Zhou Q, Munger ME, Veenstra RG, et al. Coexpression of Tim-3 and PD-1 identifies a CD8+ T-cell exhaustion phenotype in mice with disseminated acute myelogenous leukemia. Blood 2011; 117:4501–10.


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1 Set - $399.00

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Set consists of 20μg per clone.

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