Cat. No.:RF16050

TIGIT Mouse mAb Detection Set (Risk-Free™)

TIGIT Antibody: The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses (1). Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer (2).

Each Risk-Free™ monoclonal antibody set consists of several 20μg trial size mAbs with different binding features to help you find the best clone for your specific application. Go to set contents.

ProSci Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications.


Flow cytometry analysis of TIGIT over expressing HEK293 cells using (A) RF16051, (B) RF16053, (C) RF16054, (D) RF16055, (E) RF16056, (F) RF16058, and (G) control mouse IgG antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells

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Flow cytometry analysis of TIGIT over expressing HEK293 cells using (A) RF16051, (B) RF16053, (C) RF16054, (D) RF16055, (E) RF16056, (F) RF16058, and (G) control mouse IgG antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells. See high quality image.

Western blot analysis of TIGIT in over expressing HEK293 cells using RF16054, RF16055, and RF16056 antibodies at (A) 0.25 μg/ml , (B) 0.5 μg/ml, and (C) 1 μg/ml

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Western blot analysis of TIGIT in over expressing HEK293 cells using RF16054, RF16055, and RF16056 antibodies at (A) 0.25 μg/ml , (B) 0.5 μg/ml, and (C) 1 μg/ml. See high quality image.

Immunofluorescence of TIGIT in over expressing HEK293 cells using (A) RF16051, (B) RF16053, (C) RF16054, (D) RF16055, (E) RF16056, (F) RF16058, and (G) control mouse IgG antibody at 1 μg/ml

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Immunofluorescence of TIGIT in over expressing HEK293 cells using (A) RF16051, (B) RF16053, (C) RF16054, (D) RF16055, (E) RF16056, (F) RF16058, and (G) control mouse IgG antibody at 1 μg/ml. See high quality image.

Immunofluorescence of TIGIT in human stomach carcinoma tissue using (A) RF16051, (B) RF16053, (C) RF16054, (D) RF16055, (E) RF16056, (F) RF16058, and (G) control mouse IgG antibody at 5 μg/ml

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Immunofluorescence of TIGIT in human stomach carcinoma tissue using (A) RF16051, (B) RF16053, (C) RF16054, (D) RF16055, (E) RF16056, (F) RF16058, and (G) control mouse IgG antibody at 5 μg/ml. See high quality image.

Immunocytochemistry of TIGIT in over expressing HEK293 cells using (A) RF16051, (B) RF16053, (C) RF16054, (D) RF16055, (E) RF16056, (F) RF16058, and (G) control mouse IgG antibody at 1 μg/ml

(Click image to expand)
Immunocytochemistry of TIGIT in over expressing HEK293 cells using (A) RF16051, (B) RF16053, (C) RF16054, (D) RF16055, (E) RF16056, (F) RF16058, and (G) control mouse IgG antibody at 1 μg/ml. See high quality image.

Immunohistochemistry of TIGIT in human stomach carcinoma tissue (A) RF16051, (B) RF16053, (C) RF16054, (D) RF16055, (E) RF16056, (F) RF16058, and (G) control mouse IgG antibody at 2 μg/ml

(Click image to expand)
Immunohistochemistry of TIGIT in human stomach carcinoma tissue (A) RF16051, (B) RF16053, (C) RF16054, (D) RF16055, (E) RF16056, (F) RF16058, and (G) control mouse IgG antibody at 2 μg/ml. See high quality image.

Titration curve analysis of TIGIT mAbs to detect recombinant TIGIT in ELISA with RF16051, RF16053, RF16054, RF16055, RF16056 and RF16058 antibodies at decreasing concentrations

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Titration curve analysis of TIGIT mAbs to detect recombinant TIGIT in ELISA with RF16051, RF16053, RF16054, RF16055, RF16056 and RF16058 antibodies at decreasing concentrations. See high quality image.

TIGIT Mouse Monoclonal Set Contents

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TIGIT Mouse Monoclonal Set Contents.

ProSci IconSpecifications

NameTIGIT Detection Set (Risk-Free™)
Alternate Names TIGIT Antibody: T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
Host Species Mouse
Tested Applications ELISA, Flow, ICC, IF, IHC-P
Immunogen TIGIT antibodies were raised against the extracellular domain of human TIGIT.
Size 20 μg per clone

ProSci IconProperties

Clonality Monoclonal
Isotype IgG1
Conjugate Unconjugated
Physical State Liquid
Buffer PBS containing 0.02% sodium azide.
Concentration 1 mg/mL
Storage Conditions Antibodies can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ProSci IconSet Contents

Hover over application title for rating comparison image.

= Negative = Fair   = Average    = Good     = Excellent= Best Application
Cat. No.ELISAELISA Titration Curve Analysis
ELISA Titration Curve Analysis
WBWestern Blot Analysis
Western Blot Analysis
ICCICC in HEK293 Cells
ICC in HEK293 Cells
IFIF in HEK293 Cells
IF in HEK293 Cells
IHCIHC in Human Stomach Carcinoma
IHC in Human Stomach Carcinoma
FlowFlow Cytometry in HEK293 Cells
Flow Cytometry in HEK293 Cells
RF16051                      
RF16053                   
RF16054                       
RF16055                       
RF16056                        
RF16058                  

ProSci IconProduct Specific Information

ProSci's Risk-Free™ antibodies are monoclonal antibodies made to improve in vitro studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. These antibodies have the following advantages:

  • Antibodies are developed against mammalian-expressed, post-translationally modified recombinant proteins.
  • Multiple antibodies per target allows the user to choose the best antibody for their application
  • Available individually or as a set
  • Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications:
    • • ELISA• Flow Cytometry• Immunoblot • Immunohistochemistry
      • Immunocytochemistry • Immunofluorescence

ProSci IconBackground

TIGIT Antibody: The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses (1). Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer (2).


ProSci IconReferences

1) Stanietsky N, Simic H, Arapovic J, et al. The interaction of TIGIT with PVR and PVRL2 inhibits human NK cell cytotoxicity. Proc Natl Acad Sci USA 2009; 106:17858-63.
2) Johnston RJ, Comps-Agrar L, Hackney J, et al. The immunoreceptor TIGIT regulates antitumor and antiviral CD8(+) T cell effector function. Cancer Cell 2014; 26:923-37.


ANTIBODIES FOR RESEARCH USE ONLY.

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1 Set - $349.00

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Set consists of 20μg per clone.

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